School of Physical Therapy, College of Health Care, China Medical University, 91, Hsueh-Shih Rd., Taichung, Taiwan 40202, Taiwan.
Evid Based Complement Alternat Med. 2011;2011:219069. doi: 10.1093/ecam/neq005. Epub 2011 Jun 8.
The aqueous extract of Psidium guajava budding leaves (PE) bears an extremely high content of polyphenolic and isoflavonoids. Whether it could be used as an anti-tumor chemopreventive in view of anti-angiogenesis and anti-migration, we performed the assay methods including the MTT assay to examine the cell viability; the ELISA assay to test the expressions of VEGF, IL-6 and IL-8; the western blot analysis to detect TIMP-2; the gelatinolytic zymography to follow the expression of MMPs; the wound scratch assay to examine the migration capability; and the chicken chorioallantoic membrane assay to detect the suppressive angiogenesis. Results indicated that the IC50 of PE for DU145 cells was ∼0.57 mg ml(-1). In addition, PE effectively inhibited the expressions of VEGF, IL-6 and IL-8 cytokines, and MMP-2 and MMP-9, and simultaneously activated TIMP-2 and suppressed the cell migration and the angiogenesis. Conclusively, PE potentially possesses a strong anti-DU145 effect. Thus, clinically it owns the potential to be used as an effective adjuvant anti-cancer chemopreventive.
番石榴萌芽叶的水提取物（PE）含有极高含量的多酚和异黄酮。鉴于其抗血管生成和抗迁移作用，它是否可被用作抗肿瘤化学预防剂，我们进行了包括 MTT 测定法以检查细胞活力；ELISA 测定法以测试 VEGF、IL-6 和 IL-8 的表达；western blot 分析以检测 TIMP-2；明胶酶谱法以跟踪 MMPs 的表达；划痕实验以检测迁移能力；以及鸡胚尿囊膜实验以检测抑制血管生成。结果表明，PE 对 DU145 细胞的 IC50 约为 0.57mg/ml(-1)。此外，PE 有效抑制了 VEGF、IL-6 和 IL-8 细胞因子以及 MMP-2 和 MMP-9 的表达，同时激活了 TIMP-2 并抑制了细胞迁移和血管生成。总之，PE 可能具有很强的抗 DU145 作用。因此，临床上它有可能被用作有效的辅助抗癌化学预防剂。
