Institute of Plant Genetics, National Research Council, Via Amendola, 165/A, 70126, Bari, Italy.
Theor Appl Genet. 2011 Nov;123(7):1215-29. doi: 10.1007/s00122-011-1661-1. Epub 2011 Jul 29.
An integrated consensus linkage map is proposed for globe artichoke. Maternal and paternal genetic maps were constructed on the basis of an F(1) progeny derived from crossing an artichoke genotype (Mola) with its progenitor, the wild cardoon (Tolfa), using EST-derived SSRs, genomic SSRs, AFLPs, ten genes, and two morphological traits. For most genes, mainly belonging to the chlorogenic acid pathway, new markers were developed. Five of these were SNP markers analyzed through high-resolution melt technology. From the maternal (Mola) and paternal (Tolfa) maps, an integrated map was obtained, containing 337 molecular and one morphological markers ordered in 17 linkage groups (LGs), linked between Mola and Tolfa. The integrated map covers 1,488.8 cM, with an average distance of 4.4 cM between markers. The map was aligned with already existing maps for artichoke, and 12 LGs were linked via 31 bridge markers. LG numbering has been proposed. A total of 124 EST-SSRs and two genes were mapped here for the first time, providing a framework for the construction of a functional map in artichoke. The establishment of a consensus map represents a necessary condition to plan a complete sequencing of the globe artichoke genome.
提出了一种用于朝鲜蓟的综合共识连锁图谱。基于来自朝鲜蓟基因型（Mola）与其祖先野生 Cardoon（Tolfa）杂交的 F1 后代，使用 EST 衍生 SSRs、基因组 SSRs、AFLPs、10 个基因和 2 个形态特征构建了母性和父性遗传图谱。对于大多数主要属于绿原酸途径的基因，开发了新的标记。其中 5 个是通过高分辨率熔解技术分析的 SNP 标记。从母性（Mola）和父性（Tolfa）图谱中，获得了一个包含 337 个分子标记和一个形态标记的综合图谱，这些标记在 Mola 和 Tolfa 之间被排列在 17 个连锁群（LGs）中。综合图谱覆盖了 1488.8 cM，标记之间的平均距离为 4.4 cM。该图谱与已经存在的朝鲜蓟图谱进行了比对，通过 31 个桥接标记将 12 个 LG 连接起来。提出了 LG 编号。共有 124 个 EST-SSR 和两个基因首次被定位到图谱上，为朝鲜蓟构建功能图谱提供了框架。共识图谱的建立是规划朝鲜蓟全基因组测序的必要条件。
