Tang Wen-ting, Liu Xiao, Fang Min-feng, Yue Ming
Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi'an 710069, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2011 May;31(5):1220-4.
The effects of enhanced ultraviolet-B (UV-B) radiation on chemical compositions of radix, stem and leaf of Scutellaria baicalensis Georgi were studied via FTIR Results showed that alcohols and phenolic substances in the radix and leaf of S. baicalensis Georgi were increased by UV-B radiation as well as the content of flavonoids and flavonoids glycosides. The terpenoids content in radix and protein content in leaf were increased by supplementary UV-B radiation. Contrary to the circumstance in radix and leaf, contents of alcohols and phenolic, flavonoids, unsaturated fat and lactone in stem were decreased by UV-B radiation. These results indicated that there is a trade-off in distribution of the metabolites in radix, stem and leaf of different parts. More information could be revealed for the result of FTIR spectral analysis via FTIR second derivative spectrum, especially the information of the combination between flavonoids and glycosides. The effects of UV-B radiation on chemical composition in radix, stem and leaf of S. baicalensis Georgi could be examined simply and rapidly via the method of FTIR. Thus, it can be used as a prior method before the further analysis of chemical composition.
通过傅里叶变换红外光谱(FTIR)研究了增强的紫外线-B(UV-B)辐射对黄芩根、茎和叶化学成分的影响。结果表明,UV-B辐射使黄芩根和叶中的醇类和酚类物质增加,同时黄酮类和黄酮苷含量也增加。补充UV-B辐射使根中的萜类含量和叶中的蛋白质含量增加。与根和叶的情况相反,UV-B辐射使茎中的醇类、酚类、黄酮类、不饱和脂肪和内酯含量降低。这些结果表明,不同部位的根、茎和叶中代谢产物的分布存在权衡。通过FTIR二阶导数光谱可以从FTIR光谱分析结果中揭示更多信息,特别是黄酮类和糖苷结合的信息。通过FTIR方法可以简单快速地检测UV-B辐射对黄芩根、茎和叶化学成分的影响。因此,它可以作为化学成分进一步分析之前的一种优先方法。