Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentisty, Kyung Hee University, Seoul 130-701, Republic of Korea.
J Biotechnol. 2011 Sep 20;155(3):308-11. doi: 10.1016/j.jbiotec.2011.07.009. Epub 2011 Jul 20.
We developed a simple and reproducible epithelial cell-patterning tool on a PDMS (polydimethylsiloxane) polymer surface using a micro plasma structure that did not require chemical or biological treatment. The concept behind this novel approach was based on the fact that cells should easily adhere to the plasma-treated PDMS surface and not the inherent PDMS surface. The micro plasma structure consisted of copper and SU-8 photoresist on a glass substrate. A predetermined space (micro plasma chamber) was formed between the copper electrode layer on the upper part and the PDMS plate surrounded by the SU-8 photoresist structure. The single cell pattern was achieved by introducing a micro-plasma structure of 30 μm in width. Using this approach, a closed cell pattern was successfully developed using a micro chamber structure that was 200 μm in diameter with a microchannel that was 10 μm in width.
我们使用微等离子体结构在 PDMS（聚二甲基硅氧烷）聚合物表面上开发了一种简单且可重复的上皮细胞图案化工具，该结构无需进行化学或生物处理。这种新颖方法的背后的理念是基于这样一个事实，即细胞应该很容易附着在等离子体处理的 PDMS 表面上，而不是附着在固有 PDMS 表面上。微等离子体结构由玻璃基板上的铜和 SU-8 光刻胶组成。在上部的铜电极层和被 SU-8 光刻胶结构包围的 PDMS 板之间形成预定的空间（微等离子体腔室）。通过引入宽度为 30μm 的微等离子体结构，可以实现单细胞图案。使用这种方法，成功地使用直径为 200μm 的微室结构和宽度为 10μm 的微通道开发了封闭的细胞图案。
