Department of Physiology and Biochemistry, Jordan University of Science and Technology, Irbid, Jordan.
System on Chip Lab, Department of Mechanical Engineering, Khalifa University, Abu Dhabi, UAE.
PLoS One. 2022 Jun 16;17(6):e0269914. doi: 10.1371/journal.pone.0269914. eCollection 2022.
The wettability of a polymer surface plays a critical role in cell-cell interaction and behavior. The degree to which a surface is hydrophobic or hydrophilic affects the adhesion and behavior of cells. Two distinct techniques for patterning the surface wettability of a Cyclic Olefin Copolymer (COC) substrate were developed and investigated in this article for the purpose of patterning cell growth. These include oxygen plasma treatment and graphene oxide (GO) coating to alter the wettability of the COC substrate and create hydrophilic patterned regions on a hydrophobic surface. When the two techniques are compared, patterning the surface of COC using GO film results in a more stable wettability over time and increases the roughness of the patterned area. Interestingly, both developed techniques were effective at patterning the COC surface's wettability, which modulated cell adhesion and resulted in micropatterning of cell growth. The novel methods described herein can be used in the fields of cell and tissue culture as well as in the development of new biological assays.
聚合物表面的润湿性在细胞-细胞相互作用和行为中起着关键作用。表面的疏水性或亲水性程度会影响细胞的粘附和行为。本文开发并研究了两种用于对环烯烃共聚物（COC）基底表面润湿性进行图案化的独特技术，目的是对细胞生长进行图案化。这些技术包括氧等离子体处理和氧化石墨烯（GO）涂层，以改变 COC 基底的润湿性并在疏水面上形成亲水图案化区域。当比较这两种技术时，使用 GO 薄膜对 COC 表面进行图案化会导致润湿性随时间的推移更加稳定，并增加图案化区域的粗糙度。有趣的是，这两种开发的技术都能有效地对 COC 表面的润湿性进行图案化，从而调节细胞的粘附，并导致细胞生长的微图案化。本文所述的新方法可用于细胞和组织培养领域以及新生物测定方法的开发。
