Huang H S, Tan T W, Buxton D, Anderson I E, Herring A J
Moredun Research Institute, Edinburgh, Gt. Britain.
Vet Microbiol. 1990 Feb;21(4):345-51. doi: 10.1016/0378-1135(90)90006-h.
After primary infection with Chlamydia psittaci in the draining area of the popliteal lymph node, viable organisms could be isolated from the efferent lymph only before the primary immune response developed. The lymph antibody response, as assayed by the complement fixation and immunofluorescence (IF) antibody tests, showed a rise in titre that peaked approximately 2 weeks after infection. Immunoblotting revealed that antibodies produced during this period were predominantly directed against the major outer membrane protein (MOMP). In secondary infection of convalescent sheep, an elevated IF antibody titre, already present in the lymph and blood, could not be boosted. Viable organisms could not be isolated from these sheep. Antibodies produced reacted to 12-14 bands in the immunoblot profile including the MOMP band. These potentially immunoprotective antigens, particularly MOMP, should be considered as useful candidates for an improved vaccine against ovine enzootic abortion, in further investigations.
在腘淋巴结引流区域初次感染鹦鹉热衣原体后，仅在初次免疫反应形成之前，才能从输出淋巴液中分离出活的病原体。通过补体结合试验和免疫荧光（IF）抗体试验检测的淋巴抗体反应显示，滴度升高，在感染后约2周达到峰值。免疫印迹法显示，在此期间产生的抗体主要针对主要外膜蛋白（MOMP）。在恢复期绵羊的二次感染中，已经存在于淋巴液和血液中的IF抗体滴度升高，但无法进一步提高。从这些绵羊中无法分离出活的病原体。产生的抗体在免疫印迹图谱中与12 - 14条带发生反应，包括MOMP条带。在进一步的研究中，这些潜在的免疫保护性抗原，特别是MOMP，应被视为改进的羊地方性流产疫苗的有用候选物。
