Univ Paris-Sud, Institut de Génétique et Microbiologie, CNRS UMR 8621, 91405 Orsay Cedex, France.
Fungal Biol. 2011 Aug;115(8):793-802. doi: 10.1016/j.funbio.2011.06.005. Epub 2011 Jun 21.
While grafting and transplant experiments have extensively been used to study development in animals and plants, they have seldom been employed to study fungal development. Here, grafting is used to study the interplay between mycelium and multicellular fruiting bodies during maturation in the model ascomycete Podospora anserina. Data indicate that grafts need a competent mycelium to continue their ripening. Vegetative incompatibility does not prevent transplanted fructifications to undergo development. Grafting onto mutant mycelia confirmed a previous model stating that the NADPH oxidase PaNox1 is required in the developing fruiting bodies, while the MAP kinase cascade PaMpk1 is required in the mycelium. Data also show that the IDC1 protein is required not only in the developing fruiting bodies but also in the mycelium, likely because of its role in anastomosis. Finally, entry inside the grafted fruiting bodies of a ribosomal protein tagged with GFP could be detected, suggesting that cellular components are imported from the underlying mycelium during maturation.
虽然嫁接和移植实验已广泛应用于研究动植物的发育，但它们很少用于研究真菌的发育。在这里，嫁接被用来研究模型子囊菌 Podospora anserina 成熟过程中菌丝体和多细胞子实体之间的相互作用。数据表明，嫁接需要有活力的菌丝体才能继续成熟。营养体不亲和性并不能阻止移植的子实体进行发育。嫁接在突变体菌丝体上的实验证实了先前的一个模型，即 NADPH 氧化酶 PaNox1 在发育中的子实体中是必需的，而 MAP 激酶级联 PaMpk1 在菌丝体中是必需的。数据还表明，IDC1 蛋白不仅在发育中的子实体中，而且在菌丝体中也是必需的，这可能是因为它在吻合中的作用。最后，标记 GFP 的核糖体蛋白可以进入嫁接的子实体内部，这表明在成熟过程中，细胞成分是从下面的菌丝体中输入的。
