Lalucque Hervé, Malagnac Fabienne, Green Kimberly, Gautier Valérie, Grognet Pierre, Chan Ho Tong Laetitia, Scott Barry, Silar Philippe
Univ. Paris Diderot, Sorbonne Paris Cité, Laboratoire Interdisciplinaire des Energies de Demain (LIED), 75205 Paris, France.
Institute of Fundamental Sciences, Massey University, Palmerston North 4442, New Zealand.
Dev Biol. 2017 Jan 15;421(2):126-138. doi: 10.1016/j.ydbio.2016.12.016. Epub 2016 Dec 12.
Filamentous ascomycetes produce complex multicellular structures during sexual reproduction. Little is known about the genetic pathways enabling the construction of such structures. Here, with a combination of classical and reverse genetic methods, as well as genetic mosaic and graft analyses, we identify and provide evidence for key roles for two genes during the formation of perithecia, the sexual fruiting bodies, of the filamentous fungus Podospora anserina. Data indicate that the proteins coded by these two genes function cell-non-autonomously and that their activity depends upon conserved cysteines, making them good candidate for being involved in the transmission of a reactive oxygen species (ROS) signal generated by the PaNox1 NADPH oxidase inside the maturing fruiting body towards the PaMpk1 MAP kinase, which is located inside the underlying mycelium, in which nutrients are stored. These data provide important new insights to our understanding of how fungi build multicellular structures.
丝状子囊菌在有性生殖过程中产生复杂的多细胞结构。关于形成这些结构的遗传途径，人们了解甚少。在这里，我们结合经典和反向遗传学方法，以及遗传镶嵌和嫁接分析，鉴定并提供证据证明两个基因在丝状真菌嗜热栖热孢菌（Podospora anserina）的子囊壳（即有性子实体）形成过程中发挥关键作用。数据表明，这两个基因编码的蛋白质在细胞间发挥作用，其活性取决于保守的半胱氨酸，这使得它们很可能参与了由成熟子实体内的PaNox1 NADPH氧化酶产生的活性氧（ROS）信号向位于储存营养物质的下层菌丝体中的PaMpk1 MAP激酶的传递。这些数据为我们理解真菌如何构建多细胞结构提供了重要的新见解。
