Xie Ning, Ruprich-Robert Gwenaël, Chapeland-Leclerc Florence, Coppin Evelyne, Lalucque Hervé, Brun Sylvain, Debuchy Robert, Silar Philippe
Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China; Univ Paris Diderot, Sorbonne Paris Cité, Laboratoire Interdisciplinaire des Energies de Demain, 75205 Paris Cedex 13, France.
Univ Paris Descartes, Sorbonne Paris Cité, Laboratoire Interdisciplinaire des Energies de Demain, 75205 Paris Cedex 13, France.
Dev Biol. 2017 Sep 1;429(1):285-305. doi: 10.1016/j.ydbio.2017.06.017. Epub 2017 Jun 16.
The molecular pathways involved in the development of multicellular fruiting bodies in fungi are still not well known. Especially, the interplay between the mycelium, the female tissues and the zygotic tissues of the fruiting bodies is poorly documented. Here, we describe PM154, a new strain of the model ascomycetes Podospora anserina able to mate with itself and that enabled the easy recovery of new mutants affected in fruiting body development. By complete genome sequencing of spod1, one of the new mutants, we identified an inositol phosphate polykinase gene as essential, especially for fruiting body development. A factor present in the wild type and diffusible in mutant hyphae was able to induce the development of the maternal tissues of the fruiting body in spod1, but failed to promote complete development of the zygotic ones. Addition of myo-inositol in the growth medium was able to increase the number of developing fruiting bodies in the wild type, but not in spod1. Overall, the data indicated that inositol and inositol polyphosphates were involved in promoting fruiting body maturation, but also in regulating the number of fruiting bodies that developed after fertilization. The same effect of inositol was seen in two other fungi, Sordaria macrospora and Chaetomium globosum. Key role of the inositol polyphosphate pathway during fruiting body maturation appears thus conserved during the evolution of Sordariales fungi.
真菌中多细胞子实体发育所涉及的分子途径仍未完全清楚。特别是，子实体的菌丝体、雌性组织和合子组织之间的相互作用鲜有文献记载。在此，我们描述了PM154，它是模式子囊菌粪生粪壳菌的一个新菌株，能够自我交配，并且能轻松获得影响子实体发育的新突变体。通过对新突变体之一spod1进行全基因组测序，我们鉴定出一个肌醇磷酸多激酶基因是必需的，尤其是对子实体发育而言。野生型中存在且能在突变菌丝中扩散的一种因子能够诱导spod1中子实体母体组织的发育，但无法促进合子组织的完全发育。在生长培养基中添加肌醇能够增加野生型中发育的子实体数量，但对spod1无效。总体而言，数据表明肌醇和肌醇多磷酸盐不仅参与促进子实体成熟，还参与调节受精后发育的子实体数量。在另外两种真菌，大孢粪壳菌和球毛壳菌中也观察到了肌醇的相同作用。因此，肌醇多磷酸盐途径在子实体成熟过程中的关键作用在粪壳菌目真菌的进化过程中似乎是保守的。
