Laboratoire Génome et Développement des Plantes, UMR5096 CNRS-IRD-UP, University of Perpignan, 58 av. Paul Alduy, 66860 Perpignan, France.
Plant Sci. 2010 Nov;179(5):407-22. doi: 10.1016/j.plantsci.2010.07.019. Epub 2010 Aug 3.
Improvements in technology have rapidly changed the field of DNA sequencing. These improvements are boosted by bio-medical research. Plant science has benefited from this breakthrough, and a number of plant genomes are now available, new biological questions can be approached and new breeding strategies can be designed. The first part of this review aims to briefly describe the principles of the new sequencing methods, many of which are already used in plant laboratories. The second part summarizes the state of plant genome sequencing and illustrates the achievements in the last few years. Although already impressive, these results represent only the beginning of a new genomic era in plant science. Finally we describe some of the exciting discoveries in the structure and evolution of plant genomes made possible by genome sequencing in terms of biodiversity, genome expression and epigenetic regulations. All of these findings have already influenced plant breeding and biodiversity protection. Finally we discuss current trends, challenges and perspectives.
技术的进步使 DNA 测序领域发生了巨大变化。这些进步得益于生物医学研究。植物科学也从中受益，现在已经有许多植物基因组可供使用，可以提出新的生物学问题，并设计新的育种策略。本文第一部分简要描述了新测序方法的原理，其中许多方法已经在植物实验室中使用。第二部分总结了植物基因组测序的现状，并举例说明了过去几年的成就。尽管已经令人印象深刻，但这些结果仅代表了植物科学中一个新的基因组时代的开始。最后，我们描述了通过基因组测序在生物多样性、基因组表达和表观遗传调控方面对植物基因组结构和进化的一些令人兴奋的发现。所有这些发现都已经影响了植物育种和生物多样性保护。最后，我们讨论了当前的趋势、挑战和前景。
