College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu, People's Republic of China.
Colloids Surf B Biointerfaces. 2011 Nov 1;88(1):315-24. doi: 10.1016/j.colsurfb.2011.07.008. Epub 2011 Jul 13.
The surface of polyethersulfone (PES) membrane was modified by blending triblock copolymers of methoxyl poly(ethylene glycol)-polyurethane-methoxyl poly(ethylene glycol) (mPEG-PU-mPEG), which were synthesized through solution polymerization with mPEG Mns of 500 and 2000, respectively. The PES and PES/mPEG-PU-mPEG blended membranes were prepared through spin coating coupled with liquid-liquid phase separation. FTIR and (1)H NMR analysis confirmed that the triblock copolymers were successfully synthesized. The functional groups and morphologies of the membranes were studied by ATR-FTIR and SEM, respectively. It was found that the triblock copolymers were blended into PES membranes successfully, and the morphologies of the blended membranes were somewhat different from PES membrane. The water contact angles and platelet adhesion were decreased after blending mPEG-PU-mPEG into PES membranes. Meanwhile, the activated partial thromboplastin time (APTT) for the blended membranes increased. The anti-protein-fouling property and permeation property of the blended membranes improved obviously. SEM observation and 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay proved the surfaces of the blended membranes promoted human hepatocytes adhesion and proliferation better than PES membrane.
聚醚砜（PES）膜的表面通过混合嵌段共聚物甲氧基聚（乙二醇）-聚氨酯-甲氧基聚（乙二醇）（mPEG-PU-mPEG）进行改性，mPEG 的分子量分别为 500 和 2000，通过溶液聚合合成。PES 和 PES/mPEG-PU-mPEG 共混膜通过旋涂与液-液相分离相结合制备。FTIR 和 （1）H NMR 分析证实了嵌段共聚物的成功合成。通过 ATR-FTIR 和 SEM 分别研究了膜的官能团和形貌。发现嵌段共聚物成功地混入 PES 膜中，共混膜的形貌与 PES 膜有些不同。将 mPEG-PU-mPEG 混入 PES 膜后，水接触角和血小板粘附降低。同时，混合膜的激活部分凝血活酶时间（APTT）增加。共混膜的抗蛋白污染性能和渗透性能明显提高。SEM 观察和 3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐（MTT）试验证明，共混膜的表面比 PES 膜更有利于人肝细胞的粘附和增殖。
