School of Science, Shantou University, Shantou 515063, China.
Colloids Surf B Biointerfaces. 2013 Feb 1;102:354-60. doi: 10.1016/j.colsurfb.2012.07.037. Epub 2012 Aug 3.
A macromolecular biocompatible surface modifier for polyurethane (PU), namely, polyurethane-block-poly(vinylpyrrolidone) (PU-b-PVP) copolymer, was synthesized by coupling hydroxyl-terminated PVP with diisocyanate-terminated poly(ethylene glycol) using 1,6-hexamethylene diisocyanate (HDI) as coupling agent. The chemical structure and molecular weight of the synthesized copolymer were systematically characterized by nuclear magnetic resonance spectrum ((1)H NMR), Fourier transform infrared spectroscopy (FTIR) and gel permeation chromatography (GPC). The synthesized surface modifier was blended with PU and effectively increased the hydrophilicity of PU. To evaluate the hemocompatibility of the PU/PU-b-PVP blend films, the extent of platelet adsorption, plasma recalcification time and hemolysis were measured. To determine if the blend films were toxic to mammalian cells, viability of the cells cultured on the blend films was assayed. All results indicated that the blood and cell compatibilities of the PU films were improved significantly after entrapment of a few PU-b-PVP copolymers into PU.
一种聚氨酯（PU）的高分子生物相容表面改性剂，即聚氨酯嵌段聚（乙烯基吡咯烷酮）（PU-b-PVP）共聚物，是通过使用 1,6-己二异氰酸酯（HDI）作为偶联剂，将羟基封端的 PVP 与二异氰酸酯封端的聚乙二醇偶联合成的。通过核磁共振谱（1H NMR）、傅里叶变换红外光谱（FTIR）和凝胶渗透色谱（GPC）系统地对合成的共聚物的化学结构和分子量进行了表征。所合成的表面改性剂与 PU 共混，有效地提高了 PU 的亲水性。为了评估 PU/PU-b-PVP 共混膜的血液相容性，测量了血小板吸附程度、血浆复钙时间和溶血程度。为了确定共混膜是否对哺乳动物细胞有毒性，测定了在共混膜上培养的细胞的活力。所有结果表明，在将少量 PU-b-PVP 共聚物包埋到 PU 中后，PU 膜的血液相容性和细胞相容性得到了显著改善。
