Center for Vascular Biology, Department of Pathology and Laboratory Medicine, Weill Cornell Medical College, Cornell University, New York, NY 10065, USA.
Trends Mol Med. 2011 Nov;17(11):650-8. doi: 10.1016/j.molmed.2011.06.008. Epub 2011 Jul 29.
Once mRNAs are transcribed, spliced and transported to the cytoplasm, their fate is determined by the complex interplay of RNA binding proteins (RBPs) and microRNAs (miRNAs) that act on regulatory elements within the transcripts. The importance of post-transcriptional regulatory mechanisms in angiogenesis is underscored by the observation that perturbations in miRNAs and/or RBPs lead to profound phenotypic alterations in vascular development, homeostasis and disease, with current data suggesting that mRNAs for key angiogenic regulators (secreted factors and intracellular signaling intermediates) are subject to stringent post-transcriptional regulation by both RBPs and miRNAs. In addition, an intricate network of miRNAs and RBPs allow robust gene regulation in vascular cells. This review focuses on the miRNAs and RBPs which often cooperate to achieve precise spatial and temporal control of angiogenic regulatory genes.
一旦 mRNAs 被转录、剪接并运输到细胞质中，它们的命运就由 RNA 结合蛋白 (RBPs) 和 microRNAs (miRNAs) 与转录本中的调节元件之间的复杂相互作用决定。miRNAs 和/或 RBPs 的扰动会导致血管生成中的发育、稳态和疾病的显著表型改变，这突显了转录后调控机制在血管生成中的重要性，目前的数据表明，关键血管生成调节剂（分泌因子和细胞内信号中间物）的 mRNAs 受到 RBPs 和 miRNAs 的严格转录后调控。此外，miRNAs 和 RBPs 的复杂网络允许在血管细胞中进行稳健的基因调控。本综述重点介绍了经常合作以实现血管生成调节基因的精确时空控制的 miRNAs 和 RBPs。
