Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics-Theranostics, School of Pharmacy, Tianjin Medical University, Tianjin, P. R. China.
Electrophoresis. 2012 Oct;33(19-20):3021-7. doi: 10.1002/elps.201200042. Epub 2012 Sep 20.
In this paper, a molecularly imprinted polymer (MIP) coating grafted to a trimethylolpropane trimethacrylate (TRIM) core material for CEC was reported. The core monolith was prepared with a solution of 20% (w/w) TRIM in a mixture of porogen and a polymerization precursor, which can generate a stable electroosmotic flow due to the formation of ionizable groups after postpolymerization hydrolization. Graft polymerization took place on the resultant TRIM monolith with a mixture of template, methacrylic acid, and ethylene glycol dimethacrylate. Strong recognition ability (selectivity factor was 5.83) for S-amlodipine and resolution of enatiomers separation (up to 7.99) were obtained on the resulting grafted imprinted monolith in CEC mode. The influence of CEC conditions on chiral separation, including the composition of mobile phase, pH value, and the operating voltages was studied. These results suggest that the method of grafted polymerization reported here allows a rapid development of MIP monolith once core materials with desired properties are available, and is a good alternative to prepare CEC-based monolithic MIPs.
本文报道了一种接枝到三羟甲基丙烷三甲基丙烯酸酯（TRIM）核材料上的分子印迹聚合物（MIP）涂层，用于 CEC。核整体柱是通过在致孔剂和聚合前体的混合物中加入 20%（w/w）TRIM 溶液制备的，聚合后水解会形成可电离基团，从而产生稳定的电动流。接枝聚合是在生成的 TRIM 整体柱上进行的，使用模板、甲基丙烯酸和乙二醇二甲基丙烯酸酯的混合物。在 CEC 模式下，所得接枝印迹整体柱对 S-氨氯地平具有很强的识别能力（选择性因子为 5.83），对映异构体的拆分度高达 7.99。研究了 CEC 条件对手性分离的影响，包括流动相组成、pH 值和操作电压。这些结果表明，一旦获得具有所需性能的核材料，本文报道的接枝聚合方法可快速开发 MIP 整体柱，是制备基于 CEC 的整体 MIP 的一种很好的替代方法。
