Department of Biochemistry, Maharshi Dayanand University, Rohtak 124001, Haryana, India.
Biosens Bioelectron. 2011 Oct 15;28(1):64-70. doi: 10.1016/j.bios.2011.06.044. Epub 2011 Jul 6.
A new zinc oxide nanoparticles/chitosan/carboxylated multiwall carbonnanotube/polyaniline (ZnO-NPs/CHIT/c-MWCNT/PANI) composite film has been synthesized on platinum (Pt) electrode using electrochemical techniques. Three enzymes, creatinine amidohydrolase (CA), creatine amidinohydrolase (CI) and sarcosine oxidase (SO) were immobilized on ZnO-NPs/CHIT/c-MWCNT/PANI/Pt electrode to construct the creatinine biosensor. The enzyme electrode was characterized by scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy and electrochemical impedance spectroscopy (EIS). The enzyme electrode detects creatinine level as low as 0.5 μM at a signal to noise ratio of 3 within 10s at pH 7.5 and 30°C. The fabricated creatinine biosensor showed linear working range of 10-650 μM creatinine with a sensitivity of 0.030 μA μM(-1)cm(-2). The biosensor shows only 15% loss of its initial response over a period of 120 days when stored at 4°C. The fabricated biosensor was successfully employed for determination of creatinine in human blood serum.
一种新型的氧化锌纳米粒子/壳聚糖/羧基化多壁碳纳米管/聚苯胺（ZnO-NPs/CHIT/c-MWCNT/PANI）复合膜已通过电化学技术在铂（Pt）电极上合成。三种酶，肌酸酰胺水解酶（CA）、肌酸 amidinohydrolase（CI）和肌氨酸氧化酶（SO）固定在 ZnO-NPs/CHIT/c-MWCNT/PANI/Pt 电极上，构建肌酐生物传感器。酶电极的特征在于扫描电子显微镜（SEM）、傅里叶变换红外（FTIR）光谱和电化学阻抗谱（EIS）。酶电极在 pH 7.5 和 30°C 下，在 10s 内，信噪比为 3 时，可检测低至 0.5 μM 的肌酐水平。所制备的肌酐生物传感器在 10-650 μM 肌酐范围内具有线性工作范围，灵敏度为 0.030 μA μM(-1)cm(-2)。该生物传感器在 4°C 下储存 120 天时，初始响应仅损失 15%。所制备的生物传感器成功地用于测定人血清中的肌酐。
