Dias M, Newton D, McLeod G, Belch J J, Khan F
Department of Anesthesia, University of Dundee, Dundee, UK.
Int Angiol. 2011 Oct;30(5):424-8.
Calcitonin gene-related peptide (CGRP) is a potential mediator of neurogenic inflammation in eczema, psoriasis and rosacea, and also contributes to vasodilatation and oedema in complex regional pain disorder. We investigated the feasibility of administering CGRP and its antagonist CGRP8-37 by iontophoresis in human skin to characterise their vasoactive profiles.
Two doses of each peptide were administered by iontophoresis (5 and 10 min duration at 0.1 mA) to the forearm skin of 6 healthy young men. Skin blood flow responses over 25 min were assessed using laser Doppler imaging.
Iontophoresis of CGRP caused a gradual change in blood flow, with no significant difference in response between each dose. The peak change in flow had a coefficient of variation of 21% to 36%, while the variability of the total cumulative response was much greater. Iontophoresis of CGRP8-37 for 5 min caused only a small, transient increase in skin blood flow, while 10 min iontophoresis resulted in a significant increase in blood flow.
CGRP and CGRP8-37 can be administered by iontophoresis to human skin. Further experiments are needed to determine the optimum duration of iontophoresis and period of measurement.
降钙素基因相关肽（CGRP）是湿疹、银屑病和酒渣鼻神经源性炎症的潜在介质，在复杂性区域疼痛综合征中也与血管舒张和水肿有关。我们研究了通过离子电渗法将CGRP及其拮抗剂CGRP8 - 37应用于人体皮肤以表征其血管活性特征的可行性。
对6名健康年轻男性的前臂皮肤通过离子电渗法给予每种肽的两种剂量（在0.1 mA下持续5分钟和10分钟）。使用激光多普勒成像评估25分钟内的皮肤血流反应。
CGRP的离子电渗引起血流逐渐变化，各剂量之间的反应无显著差异。血流峰值变化的变异系数为21%至36%，而总累积反应的变异性则大得多。CGRP8 - 37离子电渗5分钟仅引起皮肤血流的小幅短暂增加，而10分钟离子电渗导致血流显著增加。
CGRP和CGRP8 - 37可通过离子电渗法应用于人体皮肤。需要进一步实验以确定离子电渗的最佳持续时间和测量周期。
