Yagura H, Tamaki T, Furitsu T, Tomiyama Y, Nishiura T, Tominaga N, Katagiri S, Yonezawa T, Tarui S
Second Department of Internal Medicine, Osaka University Medical School, Japan.
Blut. 1990 Mar;60(3):181-6. doi: 10.1007/BF01720273.
Functional and structural characteristics of interleukin 2 (IL-2) receptors on B-cell chronic lymphocytic leukemia (B-CLL) cells were analyzed by a proliferation assay, IL-2 binding assay and cross-linking study. In the 3H-thymidine incorporation assay, purified B-CLL cells from four out of sixteen cases, in which the percentage of Tac antigen (Tac Ag) positive cells in peripheral blood lymphocytes ranged from 0 to 48.8%, responded to IL-2 (100 U/ml) after both 3- and 6-day incubation. No relationship was found between the responsiveness to IL-2 and the percentage of Tac Ag positive cells. In the radiolabeled IL-2 binding assay, however, B-CLL cells from all seven cases examined, including three cases with mitogenic response to IL-2 and four cases without mitogenic response, were shown to have both high- and low-affinity receptors. The number of high- and low-affinity receptors per cell ranged from 29-186 and from 420 to 1,800, respectively. Furthermore, with the affinity cross-linking method p55 (Tac Ag) and p70/75 were found even in cases without mitogenic response in their B-CLL cells. In conclusion, the B-CLL cells so far examined possessed high-affinity IL-2 receptors consisting of p55 and p70/75; nevertheless, this was not sufficient to respond to the mitogenic signal of IL-2.
通过增殖试验、白细胞介素-2（IL-2）结合试验和交联研究，分析了B细胞慢性淋巴细胞白血病（B-CLL）细胞上IL-2受体的功能和结构特征。在³H-胸腺嘧啶核苷掺入试验中，从16例中的4例纯化的B-CLL细胞，其外周血淋巴细胞中Tac抗原（Tac Ag）阳性细胞百分比在0至48.8%之间，在培养3天和6天后对IL-2（100 U/ml）有反应。未发现对IL-2的反应性与Tac Ag阳性细胞百分比之间存在关联。然而，在放射性标记的IL-2结合试验中，所检测的全部7例B-CLL细胞，包括3例对IL-2有丝裂原反应的病例和4例无丝裂原反应的病例，均显示同时具有高亲和力和低亲和力受体。每个细胞的高亲和力和低亲和力受体数量分别在29至186个以及420至1800个之间。此外，采用亲和力交联方法，即使在其B-CLL细胞无丝裂原反应的病例中也发现了p55（Tac Ag）和p70/75。总之，迄今为止所检测的B-CLL细胞具有由p55和p70/75组成的高亲和力IL-2受体；然而，这并不足以对IL-2的有丝分裂原信号产生反应。
