Naruo K, Hinuma S, Kato K, Koyama M, Tada H, Shiho O, Tsukamoto K
Biochem Biophys Res Commun. 1985 Apr 16;128(1):257-64. doi: 10.1016/0006-291x(85)91672-9.
We compared the biological properties of the purified recombinant human IL-2 derived from E. coli with those of purified natural IL-2. Both had almost the same specific in vitro activities on a weight basis to support long-term proliferation of IL-2 dependent human peripheral blood lymphocytes, a mouse killer T cell line, and a mouse natural killer cell line; induce killer cells in normal mouse spleen cells; and induce antibody forming cells in nude mouse spleen cells. No differences in these biological activities were found between two forms of natural IL-2 that were separable by reverse phase high performance liquid chromatography.
我们比较了源自大肠杆菌的纯化重组人白细胞介素-2（IL-2）与纯化天然IL-2的生物学特性。二者在重量基础上具有几乎相同的体外比活性，以支持依赖IL-2的人外周血淋巴细胞、小鼠杀伤性T细胞系和小鼠自然杀伤细胞系的长期增殖；诱导正常小鼠脾细胞中的杀伤细胞；以及诱导裸鼠脾细胞中的抗体形成细胞。通过反相高效液相色谱可分离的两种天然IL-2形式之间未发现这些生物学活性存在差异。
