Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.
Arch Virol. 2011 Oct;156(10):1905-8. doi: 10.1007/s00705-011-1078-9. Epub 2011 Jul 30.
Tomato spotted wilt virus (TSWV) is well established in most countries worldwide, while it is rarely reported in China. In this report, we have determined the complete nucleotide sequence of a TSWV isolate named TSWV-YN infecting tomato in Yunnan province in southwestern China. The tripartite genome of TSWV-YN was found to consist of L, M and S RNAs of 8910, 4773 and 2970 nt, respectively. The complete genome sequence and the sequence of each genomic region of TSWV-YN from China were compared to those of four other TSWV isolates from Brazil and Korea. The phylogenetic relationship of the Chinese TSWV-YN isolate to other TSWV isolates of different geographic origin, based on the nucleotide sequences of the glycoprotein (GP) and nucleocapsid (N) genes, was also analyzed in this study.
番茄斑萎病毒（TSWV）在世界上大多数国家都已得到很好的确立，而在中国则很少有报道。在本报告中，我们确定了一种从中国云南省感染番茄的 TSWV 分离株 TSWV-YN 的完整核苷酸序列。TSWV-YN 的三分体基因组由 L、M 和 S RNA 组成，分别为 8910、4773 和 2970nt。我们将中国 TSWV-YN 分离株的完整基因组序列和每个基因组区域的序列与来自巴西和韩国的另外 4 个 TSWV 分离株的序列进行了比较。本研究还基于糖蛋白（GP）和核衣壳（N）基因的核苷酸序列，分析了基于核苷酸序列的中国 TSWV-YN 分离株与不同地理起源的其他 TSWV 分离株的系统发育关系。
