Margaria Paolo, Rosa Cristina
Department of Plant Pathology and Environmental Microbiology, The Pennsylvania State University, University Park, PA, 16802, USA.
Arch Virol. 2015 Nov;160(11):2915-20. doi: 10.1007/s00705-015-2589-6. Epub 2015 Sep 2.
We report the first complete nucleotide sequence of a tomato spotted wilt virus (genus Tospovirus, family Bunyaviridae) isolate from the United States. The tripartite genome of PA01 consisted of L, M and S RNAs of 8914, 4765 and 2984 nt, respectively. Similarity percentages in nucleotide and amino acid sequence among PA01 and previously characterized TSWV isolates are provided here. Phylogenetic analysis on the RNA-dependent RNA polymerase (RdRp) gene placed PA01 in a different clade from an isolate from Hawaii that was partially characterized previously. Evidence of two putative reassortment events in the M segment, among PA01 and isolates from South Korea, Italy and Brazil, was found by phylogenetic and recombination analysis, further supporting a role for genetic exchange among isolates of different geographic origin in TSWV evolution.
我们报道了从美国分离出的番茄斑萎病毒（番茄斑萎病毒属，布尼亚病毒科）的首个完整核苷酸序列。PA01的三分体基因组分别由长度为8914、4765和2984个核苷酸的L、M和S RNA组成。本文提供了PA01与先前已鉴定的番茄斑萎病毒分离株之间核苷酸和氨基酸序列的相似百分比。对RNA依赖的RNA聚合酶（RdRp）基因的系统发育分析表明，PA01与先前部分鉴定的来自夏威夷的一个分离株处于不同的进化分支。通过系统发育和重组分析发现，在PA01与来自韩国、意大利和巴西的分离株的M片段中存在两个假定的重配事件，这进一步支持了不同地理来源的分离株之间的基因交换在番茄斑萎病毒进化中的作用。
