Laboratoire de Physique et d'Etude des Matériaux, École Supérieure de Physique et de Chimie Industrielles, Centre National de la Recherche Scientifique UMR 8213, Université Pierre et Marie Curie, 10 rue Vauquelin, Paris 75005,France.
J Biomed Opt. 2011 Jul;16(7):076019. doi: 10.1117/1.3603847.
We describe the implementation and use of an adaptive optics loop in the imaging path of a commercial wide field microscope. We show that it is possible to maintain the optical performances of the original microscope when imaging through aberrant biological samples. The sources used for illuminating the adaptive optics loop are spectrally independent, in excitation and emission, from the sample, so they do not appear in the final image, and their use does not contribute to the sample bleaching. Results are compared with equivalent images obtained with an identical microscope devoid of adaptive optics system.
我们描述了自适应光学环在商用宽场显微镜成像路径中的实现和使用。我们表明，在对有像差的生物样本进行成像时，保持原始显微镜的光学性能是可能的。用于照明自适应光学环的光源在激发和发射时与样本在光谱上是独立的，因此它们不会出现在最终图像中，并且它们的使用不会导致样本漂白。结果与使用相同的没有自适应光学系统的显微镜获得的等效图像进行了比较。
