Department of Cell Biology, Yale School of Medicine, 333 Cedar Street, New Haven, Connecticut 06510, USA.
Opt Lett. 2013 Jun 1;38(11):1860-2. doi: 10.1364/OL.38.001860.
Stimulated emission depletion (STED) microscopy provides diffraction-unlimited resolution in fluorescence microscopy. Imaging at the nanoscale, however, requires precise alignment of the depletion and excitation laser foci of the STED microscope. We demonstrate here that adaptive optics can be implemented to automatically align STED and confocal images with a precision of 4.3 ± 2.3 nm.
受激发射耗竭（STED）显微镜在荧光显微镜中提供了无衍射限制的分辨率。然而，在纳米尺度下进行成像需要精确对准 STED 显微镜的耗竭和激发激光焦点。我们在这里证明，可以实现自适应光学，以 4.3±2.3nm 的精度自动对准 STED 和共焦图像。
