Laboratoire de Physique et d'Étude des Matériaux, Centre National de la Recherche Scientifique, UMR 8213, École Supérieure de Physique et de Chimie Industrielles, Paris, France.
Biophys J. 2011 Jun 8;100(11):2810-9. doi: 10.1016/j.bpj.2011.03.047.
We describe a wide-field fluorescence microscope setup which combines HiLo microscopy technique with the use of a two-color fluorescent probe. It allows one-shot fluorescence optical sectioning of thick biological moving sample which is illuminated simultaneously with a flat and a structured pattern at two different wavelengths. Both homogenous and structured fluorescence images are spectrally separated at detection and combined similarly with the HiLo microscopy technique. We present optically sectioned full-field images of Xenopus laevis embryos acquired at 25 images/s frame rate.
我们描述了一种宽场荧光显微镜设置，它将 HiLo 显微镜技术与使用双色荧光探针结合在一起。它允许对厚的生物移动样本进行单次荧光光学切片，该样本同时用两个不同波长的平面和结构图案照明。同质和结构荧光图像在检测时在光谱上分离，并与 HiLo 显微镜技术类似地组合。我们以 25 帧/秒的帧速率呈现了从非洲爪蟾胚胎获取的光学切片全场图像。
