Arizona State University, Department of Physics, Tempe, Arizona 85287, USA.
J Biomed Opt. 2011 Jul;16(7):078003. doi: 10.1117/1.3600771.
We report experimental results on the inactivation of encephalomyocarditis virus, M13 bacteriophage, and Salmonella typhimurium by a visible femtosecond laser. Our results suggest that inactivation of virus and bacterium by a visible femtosecond laser involves completely different mechanisms. Inactivation of viruses by a visible femtosecond laser involves the breaking of hydrogen∕hydrophobic bonds or the separation of the weak protein links in the protein shell of a viral particle. In contrast, inactivation of bacteria is related to the damage of their DNAs due to irradiation of a visible femtosecond laser. Possible mechanisms for the inactivation of viruses and bacteria are discussed.
我们报告了用可见飞秒激光灭活脑炎心肌炎病毒、M13 噬菌体和鼠伤寒沙门氏菌的实验结果。我们的结果表明，可见飞秒激光对病毒和细菌的灭活涉及完全不同的机制。可见飞秒激光对病毒的灭活涉及破坏病毒粒子蛋白壳中的氢键/疏水性键或分离弱的蛋白质连接。相比之下，细菌的失活与可见飞秒激光照射导致其 DNA 损伤有关。讨论了病毒和细菌失活的可能机制。
