Hozumi M, Tomida M, Yamamoto-Yamaguchi Y, Kasukabe T, Okabe-Kado J, Honma Y, Hayashi M
Department of Chemotherapy, Saitama Cancer Center Research Institute, Japan.
Ciba Found Symp. 1990;148:25-33; discussion 33-42. doi: 10.1002/9780470513880.ch3.
We have purified and characterized several protein factors that regulate the growth and differentiation of mouse myeloid leukaemia M1 cells. The differentiation factor (D-factor) from conditioned medium (CM) of Ehrlich ascites tumour cells is a glycoprotein of Mr 40,000-50,000. Its amino acid sequence was found to be almost identical to that of leukaemia inhibitory factor (LIF) from Krebs II ascites cells. The differentiation inhibitory factor (I-factor) from the CM of variant M1 cell clones which were resistant to several differentiation inducers is a basic protein of apparent Mr 68,000. The growth inhibitory factor (GI-factor) that specifically inhibits the partially differentiated and still growing monocytic leukaemia M1 cells was isolated from the CM of a clone of M1 cells resistant to the differentiation inducers. This GI-factor is a basic protein with an Mr of 25,000. Regulation by these protein factors together with other known cytokines of growth and differentiation of M1 cells is reported.
我们已经纯化并鉴定了几种调节小鼠髓性白血病M1细胞生长和分化的蛋白质因子。来自艾氏腹水瘤细胞条件培养基（CM）的分化因子（D因子）是一种分子量为40,000 - 50,000的糖蛋白。发现其氨基酸序列与来自克雷布斯II腹水细胞的白血病抑制因子（LIF）几乎相同。来自对几种分化诱导剂有抗性的变体M1细胞克隆的CM的分化抑制因子（I因子）是一种表观分子量为68,000的碱性蛋白。从对分化诱导剂有抗性的M1细胞克隆的CM中分离出特异性抑制部分分化且仍在生长的单核细胞白血病M1细胞的生长抑制因子（GI因子）。这种GI因子是一种分子量为25,000的碱性蛋白。本文报道了这些蛋白质因子与其他已知细胞因子对M1细胞生长和分化的调节作用。
