Laboratoire de Biologie de la Nutrition EA 4466, Faculté des Sciences Pharmaceutiques et Biologiques, Université Paris Descartes, Sorbonne Paris Cité, Paris, France.
J Neurotrauma. 2012 Jan 20;29(2):335-42. doi: 10.1089/neu.2011.1862. Epub 2011 Oct 24.
Infectious complications are responsible for 10-25% of mortality in head-injured patients. In the present work we developed a model of infectious complications in head-injury rats using Escherichia coli (E. coli) with a stable copy of the lux operon, and monitored the infection in vivo by optical imaging. Rats were randomized into three groups: AL (healthy rats), HI (head-injury rats), and HI-EC (HI rats+single enteral bolus of E. coli, 1.3×10(9)/rat given 2 days after HI). Infection was evaluated with a camera at 2 and 6 h after E. coli challenge. Blood and organs were sampled to assess biological parameters. HI was associated with body weight loss, muscle atrophy, and plasma amino acid disturbances, in particular glutamine depletion (AL 919±37 versus HI 647±25 and HI-EC 717±20 μmol/L; p<0.05). In the HI-EC rats, the luminescence signal was observed at T+2 (mean [range]: 34,778 cpm [1617-2,918,810]), and was significantly decreased at T+6 (0 cpm [0-847,922]; p<0.05). Bacterial challenge was associated with a specific body weight loss and a decrease in gastrocnemius protein content, in alanine (AL 512±41 versus HI-EC 395±29 μmol/L; p<0.05), and in sulfur plasma amino acids. In conclusion, we propose a controlled model of HI with infectious complications characterized by specific metabolic alterations. Combined with the in vivo monitoring of the infection by bioluminescence, this model offers a valuable tool to evaluate specific strategies for HI patients.
感染并发症是颅脑损伤患者死亡的 10-25%的原因。在本工作中，我们使用带有稳定 lux 操纵子的大肠杆菌（E. coli）建立了颅脑损伤大鼠感染并发症模型，并通过光学成像对体内感染进行监测。大鼠随机分为三组：AL（健康大鼠）、HI（颅脑损伤大鼠）和 HI-EC（颅脑损伤大鼠+单剂量肠内大肠杆菌，颅脑损伤后 2 天给予 1.3×10(9)/大鼠）。在大肠杆菌攻击后 2 和 6 小时使用相机评估感染。采集血液和器官样本以评估生物学参数。HI 与体重减轻、肌肉萎缩和血浆氨基酸紊乱有关，特别是谷氨酰胺耗竭（AL 919±37 与 HI 647±25 和 HI-EC 717±20μmol/L 相比；p<0.05）。在 HI-EC 大鼠中，在 T+2 时观察到发光信号（平均值[范围]：34,778 cpm [1617-2,918,810]），在 T+6 时显著降低（0 cpm [0-847,922]；p<0.05）。细菌攻击与特定的体重减轻和比目鱼肌蛋白质含量下降有关，与丙氨酸（AL 512±41 与 HI-EC 395±29μmol/L 相比；p<0.05）和含硫血浆氨基酸有关。总之，我们提出了一种具有感染并发症的颅脑损伤的可控模型，其特征是存在特定的代谢改变。该模型结合了生物发光的体内感染监测，为评估颅脑损伤患者的特定策略提供了有价值的工具。
