EA4065 Ecosystème Intestinal, Probiotiques, Antibiotiques, Faculté de Pharmacie de Paris, Université Paris Descartes, Sorbonne Paris Cité, Paris, France.
EA4065 Ecosystème Intestinal, Probiotiques, Antibiotiques, Faculté de Pharmacie de Paris, Université Paris Descartes, Sorbonne Paris Cité, Paris, France.
Nutrition. 2018 Jan;45:104-107. doi: 10.1016/j.nut.2017.06.026. Epub 2017 Jul 8.
Head injury (HI) induces a hypercatabolic state, dysimmunity, and septic complications that increase morbidity and mortality. Although compromised immune function is usually incriminated in infection occurrence, gut dysbiosis could also be involved in this phenomenon and, to our knowledge, has never been considered. To assess if HI could affect microbiota, we explored the impact of HI on intestinal microbiota in a rodent model of fluid percussion.
Nineteen rats were randomly assigned to two groups: Healthy rats fed ad libitum (n = 7) and HI rats (n = 12), which received standard enteral nutrition for 4 d. Four days after HI, rats were euthanized and cecal contents were sampled. Cecal microbiota was assessed using real-time quantitative polymerase chain reaction.
HI significantly decreased the cecal content of strict anaerobic groups, Bacteroides/Prevotella group (HI 8.9 versus healthy controls 9.3 median log colony forming units [CFU]/g, P = 0.007), Clostridium cluster XIVab (HI 7.9 versus healthy controls 8.9 median log CFU/g, P = 0.002), Lactobacillus/Leuconostoc group (HI 8.5 versus healthy controls 9.4 median log CFU/g, P = 0.044), and Bifidobacterium sp. (HI 3.0 versus healthy controls 8.2 median log CFU/g, P < 0.001). In contrast, colonization by Escherichia coli was dramatically increased (HI 10.5 versus healthy controls 7.0 median log CFU/g, P < 0.001).
HI profoundly modified the gut microbiota homeostasis and thus could contribute to infection in head trauma patients. These preliminary results open a new field of research in the management of patients with HI.
头部损伤（HI）会引起代谢亢进状态、免疫功能障碍和感染并发症，从而增加发病率和死亡率。尽管免疫功能受损通常与感染的发生有关，但肠道菌群失调也可能与此现象有关，但据我们所知，这一点尚未得到考虑。为了评估 HI 是否会影响微生物群，我们在液压冲击的啮齿动物模型中探索了 HI 对肠道微生物群的影响。
19 只大鼠被随机分配到两组：自由进食的健康大鼠（n=7）和 HI 大鼠（n=12），它们接受标准肠内营养 4 天。HI 后 4 天，大鼠被安乐死，采集盲肠内容物。使用实时定量聚合酶链反应评估盲肠微生物群。
HI 显著降低了盲肠内容物中严格厌氧菌、拟杆菌/普雷沃氏菌组（HI 8.9 与健康对照组 9.3 中位数对数菌落形成单位[CFU]/g，P=0.007）、梭状芽胞杆菌簇 XIVab（HI 7.9 与健康对照组 8.9 中位数对数 CFU/g，P=0.002）、乳杆菌/肠球菌组（HI 8.5 与健康对照组 9.4 中位数对数 CFU/g，P=0.044）和双歧杆菌属（HI 3.0 与健康对照组 8.2 中位数对数 CFU/g，P<0.001）。相反，大肠杆菌的定植显著增加（HI 10.5 与健康对照组 7.0 中位数对数 CFU/g，P<0.001）。
HI 严重改变了肠道微生物群的平衡，从而可能导致头部创伤患者感染。这些初步结果为 HI 患者的管理开辟了一个新的研究领域。
