Instituto de Química, Universidade Federal de Uberlândia, Uberlândia, MG, Brazil.
Talanta. 2011 Sep 15;85(3):1274-8. doi: 10.1016/j.talanta.2011.06.008. Epub 2011 Jun 12.
We report here, for the first time, application of batch injection analysis (BIA) with amperometric detection for determination of the phenolic antioxidant butylated hydroxyanisole (BHA) in biodiesel. A sample plug was directly injected onto a boron-doped diamond electrode immersed in 50% v/v hydroethanolic solution with 0.1 mol L(-1) HClO(4) using an electronic micropipette. Importantly, the only preparation step required for biodiesel analysis is dilution in the same hydroethanolic electrolyte solution. Our proposed method has several advantages for routine biodiesel analysis, including: a low relative standard deviation between injections (0.29%, n=20), high analytical frequency (120 h(-1)), adequate recovery values (93-101%) for spiked samples, satisfactory accuracy (based on comparative determinations by high-performance liquid-chromatography), and a low detection limit (100 ng of BHA per g of biodiesel). Finally, our method can be adapted for the determination of other antioxidants in biodiesel samples.
我们首次报告了批量注射分析（BIA）与安培检测在生物柴油中酚类抗氧化剂丁基羟基茴香醚（BHA）测定中的应用。使用电子微量进样器，将样品直接注入到浸入 50%（v/v）羟乙基溶液中的掺硼金刚石电极中，其中含有 0.1 mol L(-1) HClO(4)。重要的是，生物柴油分析所需的唯一制备步骤是在相同的羟乙基电解质溶液中稀释。我们提出的方法在常规生物柴油分析中有几个优点，包括：注入之间的相对标准偏差低（0.29%，n=20），分析频率高（120 h(-1)），加标样品的回收率值适中（93-101%），准确度令人满意（基于高效液相色谱法的比较测定），检测限低（每克生物柴油 100 纳克 BHA）。最后，我们的方法可以适用于生物柴油样品中其他抗氧化剂的测定。
