Gjerde H, Christophersen A S, Skuterud B, Klemetsen K, Mørland J
National Institute of Forensic Toxicology, Oslo, Norway.
Forensic Sci Int. 1990 Feb;44(2-3):179-85. doi: 10.1016/0379-0738(90)90248-w.
A screening method for the detection of drugs in haemolysed whole blood has been evaluated. Methanolic extracts of 300 forensic blood samples known to be positive or negative for drugs were analysed with EMIT d.a.u. assay kits for amphetamine, cannabinoids, opiates and benzodiazepines (the latter to analyse for diazepam and the main metabolite N-desmethyldiazepam). There were very few false positive results, except for the amphetamine assay in postmortem blood samples, where 9% were false positive. For amphetamine and cannabinoids a few false negatives were found, these were from samples with very low drug concentrations. No false negatives were found for opiates and diazepam. The present modification of the EMIT d.a.u. method seems to be a good method for screening of drugs in forensic blood samples, except for amphetamine in postmortem samples. The method is simple and requires only 0.5 ml blood.
一种用于检测溶血全血中药物的筛查方法已得到评估。对300份已知药物呈阳性或阴性的法医血液样本的甲醇提取物，使用EMIT d.a.u.检测试剂盒分析苯丙胺、大麻素、阿片类药物和苯二氮䓬类药物（后者用于分析地西泮及其主要代谢物N -去甲基地西泮）。除了死后血液样本中的苯丙胺检测有9%为假阳性外，假阳性结果很少。对于苯丙胺和大麻素，发现了一些假阴性结果，这些来自药物浓度非常低的样本。阿片类药物和地西泮未发现假阴性结果。EMIT d.a.u.方法的当前改进版本似乎是筛查法医血液样本中药物的好方法，但死后样本中的苯丙胺检测除外。该方法简单，仅需0.5毫升血液。
