采用荧光偏振免疫分析（FPIA）和酶增强免疫测定技术（EMIT-dau）免疫分析法测定全血中滥用药物的比较研究。

The determination of drugs of abuse in whole blood by means of FPIA and EMIT-dau immunoassays--a comparative study.

作者信息

Bogusz M, Aderjan R, Schmitt G, Nadler E, Neureither B

机构信息

Institute of Forensic Medicine, Karl Ruprecht University, Heidelberg, F.R.G.

出版信息

Forensic Sci Int. 1990 Nov;48(1):27-37. doi: 10.1016/0379-0738(90)90269-5.

DOI:10.1016/0379-0738(90)90269-5

PMID:2279719

Abstract

Six groups of common drugs of abuse (cannabinoids, benzoylecgonine, opiates, barbiturates, benzodiazepines and amphetamines) were determined in whole blood after acetone precipitation, using enzyme multiplied immunoassay (EMIT dau) and fluorescence polarisation immunoassay (FPIA--Abbott TDx and ADx) methods. Both methods, designed primarily for urine, allowed the determination of all above mentioned class of drugs but amphetamine. Only 1 ml of a pre- or postmortem blood sample was needed. The sensitivity of cannabinoids determination was higher by FPIA. The FPIA method gave more precise results, particularly in the case of autopsy blood. The method was applied for drug screening in autopsy and police blood samples. The results (both positive and negative) were in agreement with those obtained with chromatographic methods.

摘要

采用酶放大免疫分析技术（EMIT dau）和荧光偏振免疫分析技术（FPIA——雅培TDx和ADx），通过丙酮沉淀法对全血中的六类常见滥用药物（大麻素、苯甲酰爱康宁、阿片类药物、巴比妥类药物、苯二氮卓类药物和苯丙胺）进行了测定。这两种主要用于尿液检测的方法，能够测定除苯丙胺外上述所有类别的药物。仅需1毫升生前或死后血液样本。FPIA测定大麻素的灵敏度更高。FPIA方法得出的结果更精确，尤其是在尸检血液检测中。该方法应用于尸检和警方血液样本的药物筛查。结果（阳性和阴性）与色谱法所得结果一致。