Lewellen L J, McCurdy H H
Division of Forensic Sciences, Decatur, Georgia 30037.
J Anal Toxicol. 1988 Sep-Oct;12(5):260-4. doi: 10.1093/jat/12.5.260.
This paper describes a procedure for the analysis of whole blood by six commonly used EMIT d.a.u. assays: barbiturates, benzodiazepines, cocaine metabolite, opiates, amphetamines, and the cannabinoid 20 assays using the Syva Autocarousel and the COBAS MIRA. The sensitivity of the method rivals that of other immunoassay methods, such as RIA, for the detection of drugs in blood. The procedure has been successfully applied to the analysis of several hundred postmortem forensic blood specimens.
本文介绍了一种使用Syva自动转盘和COBAS MIRA,通过六种常用的酶放大免疫测定技术(EMIT d.a.u.)分析全血的方法:巴比妥类药物、苯二氮卓类药物、可卡因代谢物、阿片类药物、苯丙胺类药物以及大麻素类药物的20种测定方法。该方法的灵敏度可与其他免疫测定方法(如放射免疫分析,RIA)相媲美,用于检测血液中的药物。该方法已成功应用于数百份法医尸检血液标本的分析。