Anand Swarup Kolla R L, Sattar Munavvar A, Abdullah Nor A, Abdulla Mohammed H, Salman Ibrahim M, Rathore Hassaan A, Johns Edward J
Physiology Research Lab, School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden - 11800, Penang.
Pharmacognosy Res. 2010 Jan;2(1):31-5. doi: 10.4103/0974-8490.60582.
Cardiovascular complications are consistently observed in diabetic patients across all age groups. The objective of the present study was to investigate the effect of aqueous extract of the fruit pulp of Hylocereus undatus (DFE) on aortic stiffness and oxidative stress in streptozotocin (STZ)-induced diabetes in rats. Twenty-four male, Sprague-Dawley rats were randomized into four groups: I (control), II (diabetic), III (DFE, 250 mg/kg) and IV (DFE 500 mg/kg). Diabetes was induced in groups II, III and IV by intraperitoneal (i.p.) injection of STZ (40 mg/kg). After confirmation of diabetes, group III and IV received DFE for 5 weeks. Pulse wave velocity (PWV) was used as a marker of aortic stiffness and was determined at the end of 5 weeks. DFE significantly decreased (P < 0.05) the fasting blood glucose levels in diabetic rats, but not to normal levels. Systolic blood pressure, pulse pressure and PWV were significantly increased (P < 0.05) in diabetic rats at the end of 5 weeks in comparison with control group. DFE treatment significantly decreased (P < 0.05) these elevations. Oxidative damage was observed in group II after 5 weeks. Plasma malondialdehyde levels significantly decreased (P < 0.05), while superoxide dismutase and total antioxidant capacity significantly increased (P < 0.05) with DFE treatment in comparison with group II. These data demonstrate that DFE treatment was effective in controlling oxidative damage and decreasing the aortic stiffness measured by PWV in STZ-induced diabetes in rats.
在所有年龄组的糖尿病患者中均持续观察到心血管并发症。本研究的目的是调查火龙果果肉水提取物（DFE）对链脲佐菌素（STZ）诱导的糖尿病大鼠主动脉僵硬度和氧化应激的影响。将24只雄性Sprague-Dawley大鼠随机分为四组：I组（对照组）、II组（糖尿病组）、III组（DFE 250 mg/kg）和IV组（DFE 500 mg/kg）。通过腹腔注射STZ（40 mg/kg）诱导II、III和IV组患糖尿病。确认糖尿病后，III组和IV组接受DFE治疗5周。脉搏波速度（PWV）用作主动脉僵硬度的指标，并在5周结束时进行测定。DFE显著降低（P<0.05）糖尿病大鼠的空腹血糖水平，但未降至正常水平。与对照组相比，糖尿病大鼠在5周结束时收缩压、脉压和PWV显著升高（P<0.05）。DFE治疗显著降低了（P<0.05）这些升高水平。5周后在II组中观察到氧化损伤。与II组相比，DFE治疗使血浆丙二醛水平显著降低（P<0.05），而超氧化物歧化酶和总抗氧化能力显著增加（P<0.05）。这些数据表明，DFE治疗可有效控制氧化损伤，并降低STZ诱导的糖尿病大鼠中通过PWV测量的主动脉僵硬度。
