Wybraniec Sławomir, Mizrahi Yosef
Department of Chemical Engineering and Technology, Institute C-1, Faculty of Analytical Chemistry, Cracow University of Technology, ul. Warszawska 24, Cracow 31-155, Poland.
J Agric Food Chem. 2002 Oct 9;50(21):6086-9. doi: 10.1021/jf020145k.
Determination of profiles and total contents of betacyanins in cactus fruits of Hylocereus species using chromatographic and spectrophotometric method is described. The investigated species were H. polyrhizus, H. purpusii, H. costaricensis, H. sp. 487 (all red-flesh species and hybrids made among them), and the white- or red-flesh species H. undatus. Hybrids included hybrid 1 (H. undatus white-flesh clone and H. sp. 487), hybrid 35 (H. sp. 487 and H. polyrhizus), and the reciprocal hybrid hybrid 95 (H. polyrhizus and H. sp. 487). Fruits of H. polyrhizus exhibited the highest relative concentration (expressed as percentage of the total HPLC peak area) of hylocerenin, a recently discovered pigment, and a high relative concentration of phyllocactin. Hylocerenin and isohylocerenin, present in fruits at relative concentrations of 11.7 and 5.8%, respectively, are probably responsible for the fluorescent color of the fruit pulp. H. costaricensis fruits have a much higher content of phyllocactin (63.9%), which is almost 4 times higher than the betanin content. These differences in pigment concentrations might explain the differences in red hues of the flesh of these fruits.
描述了使用色谱法和分光光度法测定量天尺属仙人掌果实中甜菜色素的谱图和总含量。所研究的物种有多根量天尺、普尔皮量天尺、哥斯达黎加量天尺、量天尺 sp. 487（所有红肉品种及其间杂交品种），以及白肉或红肉品种的火龙果。杂交品种包括杂交 1（火龙果白肉克隆体和量天尺 sp. 487）、杂交 35（量天尺 sp. 487 和多根量天尺），以及反交杂交品种杂交 95（多根量天尺和量天尺 sp. 487）。多根量天尺的果实中，最近发现的色素hylocerenin的相对浓度（以总高效液相色谱峰面积的百分比表示）最高，并且叶仙人掌红素的相对浓度也很高。果实中hylocerenin和异hylocerenin的相对浓度分别为11.7%和5.8%，它们可能是果肉荧光色的原因。哥斯达黎加量天尺果实中叶仙人掌红素的含量高得多（63.9%），几乎是甜菜红素含量的4倍。这些色素浓度的差异可能解释了这些果实果肉红色调的差异。
