Department of the Surgery, Jinan Central Hospital of Shandong University, Jinan, China.
J Cancer Res Clin Oncol. 2011 Oct;137(10):1419-24. doi: 10.1007/s00432-011-1011-3. Epub 2011 Aug 2.
The present study aims to investigate the expression and significance of the anti-apoptotic gene Bag-1 in colorectal cancer and to evaluate the relationship between the gene and the disease.
Bag-1 expression was examined in 320 colorectal cancer and 30 normal colorectal tissue samples using reverse transcriptase polymerase chain reaction (RT-PCR) and the immunohistochemical staining (streptavidin-biotin-peroxidase complex method.
Using RT-PCR, Bag-1 was observed to be expressed in colorectal cancer tissues, but not in normal colorectal tissues. The expression of Bag-1 in colorectal cancer was closely correlated with pathologic grade, distant metastasis, Dukes stage, and prognosis, but it was not correlated with the pathologic type, tumor diameter, depth of invasion, and lymph node metastasis.
Bag-1 protein was found to be overexpressed in colorectal cancer. They might be regarded as a biomarker for the diagnosis of the early stages of colorectal cancer. In addition, they have particular significance for the prognosis of colorectal cancer.
本研究旨在探讨凋亡抑制基因 Bag-1 在结直肠癌中的表达及其意义，并评估该基因与疾病的关系。
采用逆转录聚合酶链反应（RT-PCR）和免疫组织化学染色（链亲和素-生物素-过氧化物酶复合物法）检测 320 例结直肠癌及 30 例正常结直肠组织中 Bag-1 的表达。
RT-PCR 结果显示 Bag-1 在结直肠癌组织中表达，而在正常结直肠组织中不表达。Bag-1 在结直肠癌中的表达与病理分级、远处转移、Dukes 分期和预后密切相关，而与病理类型、肿瘤直径、浸润深度和淋巴结转移无关。
Bag-1 蛋白在结直肠癌中过度表达。它们可能被视为结直肠癌早期诊断的标志物。此外，它们对结直肠癌的预后具有特殊意义。
