Stuurman N, Meijne A M, van der Pol A J, de Jong L, van Driel R, van Renswoude J
E.C. Slater Institute for Biochemical Research, University of Amsterdam, The Netherlands.
J Biol Chem. 1990 Apr 5;265(10):5460-5.
We compared the protein composition of the nuclear matrix isolated from several murine embryonal carcinoma cells and mature tissues by two-dimensional gel electrophoresis. Two nuclear matrix fractions were investigated: the "peripheral" nuclear matrix (matrix proteins that remain insoluble after reduction), and the "internal" nuclear matrix (matrix proteins released by reduction). The two subfractions have completely different protein compositions. Although numerous differences in nuclear matrix protein composition among different cell types were observed, a limited set of polypeptides common to all mouse cell types was identified. A majority of these common proteins was also present in cells from other mammalian species (i.e. rat and human). For this set of proteins, we coin the term "minimal matrix." As expected, lamin B, known to be expressed throughout differentiation, is part of the common set of peripheral nuclear matrix proteins. Lamins A and C are not because these proteins were absent from undifferentiated embryonal carcinoma cells. Since these common nuclear matrix proteins occur in all mammalian nuclear matrices analyzed so far, it is likely that they have a basic role in nuclear organization and function.
我们通过二维凝胶电泳比较了从几种小鼠胚胎癌细胞和成熟组织中分离出的核基质的蛋白质组成。研究了两种核基质组分：“外周”核基质（还原后仍不溶的基质蛋白）和“内部”核基质（还原后释放的基质蛋白）。这两个亚组分具有完全不同的蛋白质组成。尽管观察到不同细胞类型之间核基质蛋白质组成存在许多差异，但鉴定出了一组所有小鼠细胞类型共有的有限多肽。这些常见蛋白质中的大多数也存在于其他哺乳动物物种（即大鼠和人类）的细胞中。对于这组蛋白质，我们创造了“最小基质”这个术语。正如预期的那样，已知在整个分化过程中都有表达的核纤层蛋白B是外周核基质共同蛋白质组的一部分。核纤层蛋白A和C则不是，因为这些蛋白质在未分化的胚胎癌细胞中不存在。由于这些常见的核基质蛋白存在于迄今为止分析的所有哺乳动物核基质中，它们很可能在核组织和功能中具有基本作用。
