Yan Guanghai, Jin Guangyu, Li Liangchang, Qin Xiangzheng, Zheng Changji, Li Guangzhao
Department of Anatomy, Yanbian University School of Basic Medical Sciences, Yanji 133002, China.
Zhongguo Zhong Yao Za Zhi. 2011 Apr;36(8):1067-70.
To explore the protective effects and mechanism of ethanol extract of Inonotus obliquus (EEIO) injection on asthmatic mice.
OVA was injected intraperitoneally and inhaled to produce the asthmatic model. Thirty two mice were randomly divided into four groups: control group, asthma group and I. obliquus groups of high and low dose. The concentrations of IL-4, IL-5, IL-13 and IFN-gamma in BALF, the phosphor-p38 MAPK in lung tissues were respectively measured by ELISA and Western blotting. The number of inflammatory cells in BALF and histopathology changes were observed.
In asthmatic group, the number of inflammatory cells and the concentrations of IL-4, IL-5, IL-13 in BALF and phospho-p38 MAPK in lung tissue were higher, while IFN-gamma were lower than those in normal control mice (P < 0.05). In I. obliquus group, the number of inflammatory cells, the concentrations of IL-4, IL-5, IL-13 in BALF and phosphor-p38 MAPK in lung tissue were lower, but were higher than those in normal control mice (P < 0.05), and histropathology damage was alleviated significantly. There was no significant difference observed among the efficacies in the I. obliquus groups of high and low dose.
p38 MAPK may play a role in pathological process of asthma. I. obliquus effectively treats asthma by inhibiting the expression of phosphor-p38 MAPK, correcting the unbalance of IFN-gamma/IL-4 and decreasing the number of inflammatory cells.
探讨桦褐孔菌乙醇提取物（EEIO）注射液对哮喘小鼠的保护作用及机制。
通过腹腔注射和雾化吸入卵清蛋白（OVA）制备哮喘模型。将32只小鼠随机分为四组：对照组、哮喘组、桦褐孔菌高剂量组和低剂量组。采用酶联免疫吸附测定法（ELISA）和蛋白质免疫印迹法（Western blotting）分别检测支气管肺泡灌洗液（BALF）中白细胞介素-4（IL-4）、白细胞介素-5（IL-5）、白细胞介素-13（IL-13）和干扰素-γ（IFN-γ）的浓度，以及肺组织中磷酸化p38丝裂原活化蛋白激酶（phosphor-p38 MAPK）的表达。观察BALF中炎症细胞数量及组织病理学变化。
哮喘组BALF中炎症细胞数量、IL-4、IL-5、IL-13浓度及肺组织中phosphor-p38 MAPK表达高于正常对照组，IFN-γ浓度低于正常对照组（P<0.05）。桦褐孔菌组BALF中炎症细胞数量、IL-4、IL-5、IL-13浓度及肺组织中phosphor-p38 MAPK表达低于哮喘组，但高于正常对照组（P<0.05），组织病理学损伤明显减轻。桦褐孔菌高、低剂量组疗效差异无统计学意义。
p38 MAPK可能参与哮喘的病理过程。桦褐孔菌可通过抑制phosphor-p38 MAPK表达、纠正IFN-γ/IL-4失衡及减少炎症细胞数量有效治疗哮喘。
