Gashnikova N M, Safronov P F, Nikonorova Yu V, Unagaeva N V, Lapteva T A, Bogachev V V, Baryshev P B, Totmenin A V, Bukin E K, Bocharov E F, Chernousova N Ia, Stavskiĭ E A
Zh Mikrobiol Epidemiol Immunobiol. 2011 May-Jun(3):38-43.
Study of circulating 02_AG recombinant form HIV-1 isolates that have been rapidly spreading in Novosibirsk region during 3 recent years.
WHO protocol for primary HIV isolation was used, automatic sequencer was used for genetic characterization of isolates. Virus specific RNA were isolated and env HIV-1 region DNA fragments were processed. Phylogenetic analysis was also performed.
CRF_02AG HIV-1 isolated from peripheral blood of HIV-1 positive patients belonged to CCR5 tropic viruses and had various reproduction characteristics. Most of the HIV isolated were rapidly replicating virus variants characterized by an ability to accumulate high levels of virus protein p24 in cultural fluid. Infectivity and reproductive properties of HIV isolates were confirmed in experimental infection by using clarified cultural liquid of mononuclear cells from healthy donors. Phylogenetic analysis of CRF02_AG HIV-1 variants isolated in Novosibirsk region in 2007 - 2010 showed the formation of a separate outbreak in the area caused by emergence of CRF02_AG HIV-1 in human population.
A collection of genetically and biologically characterized CRF02_AG HIV-1 isolates that has not been spreading previously in Russia.
研究近年来在新西伯利亚地区迅速传播的循环型02_AG重组形式HIV-1分离株。
采用世界卫生组织原发性HIV分离方案，使用自动测序仪对分离株进行基因特征分析。分离病毒特异性RNA并处理HIV-1 env区域DNA片段，同时进行系统发育分析。
从HIV-1阳性患者外周血中分离出的CRF_02AG HIV-1属于CCR5嗜性病毒，具有多种复制特征。大多数分离出的HIV是快速复制的病毒变体，其特点是能够在培养液中积累高水平的病毒蛋白p24。通过使用健康供体单核细胞的澄清培养液进行实验性感染，证实了HIV分离株的感染性和复制特性。对2007 - 2010年在新西伯利亚地区分离出的CRF02_AG HIV-1变体进行的系统发育分析表明，该地区因人群中出现CRF02_AG HIV-1而形成了一个单独的疫情爆发。
收集了一组在俄罗斯此前未曾传播过的经基因和生物学特征鉴定的CRF02_AG HIV-1分离株。
