Smart G J, Wilson M, Davies E H, Kieser J B
Department of Periodontology, Institute of Dental Surgery, London, UK.
J Clin Periodontol. 1990 Mar;17(3):174-8. doi: 10.1111/j.1600-051x.1990.tb01082.x.
Recent demonstrations of the superficial nature and ease of removal of root-surface-associated cytotoxic materials suggest that extensive root-surface instrumentation is not warranted clinically. This in vitro investigation determined the detoxifying effects of a conservative regime of ultrasonic root debridement, using the Limulus amoebocyte lysate assay for lipopolysaccharide (LPS) as an indication of the presence or absence of cytotoxic materials. 20 extracted periodontally-involved single-rooted teeth, with no clinically detectable calculus deposits, were debrided with a Cavitron TF-10 tip. Light pressure, resulting from a force application of approximately 50 g, lasting for 0.8 s/mm2 of root surface was used and complete overlapping instrumentation ensured. The finding of LPS levels of less than 2.5 ng per root in 19 out of 20 teeth after such debridement was comparable to LPS levels found on healthy, uninvolved control teeth, and thus endorses the growing belief that root surface cleanliness can be readily achieved.
近期有关牙根表面相关细胞毒性物质具有表面性且易于去除的证明表明，临床上无需进行广泛的牙根表面器械操作。这项体外研究使用鲎试剂检测法检测脂多糖（LPS），以此作为细胞毒性物质存在与否的指标，确定了保守的超声牙根清创术的解毒效果。选取20颗因牙周问题拔除的单根牙，这些牙齿临床上未检测到牙结石沉积，使用Cavitron TF-10尖端进行清创。施加约50克的力，持续0.8秒/平方毫米的牙根表面，采用轻压力，并确保器械操作完全重叠。清创后，20颗牙齿中有19颗牙根的LPS水平低于每根2.5纳克，这与健康未受累对照牙齿上发现的LPS水平相当，因此支持了人们越来越相信的观点，即牙根表面清洁很容易实现。
