Department of Pulmonary Medicine and Biomedical Research Center, Zhongshan Hospital, Fudan University, Shanghai, China.
J Cell Mol Med. 2011 Oct;15(10):2262-8. doi: 10.1111/j.1582-4934.2011.01404.x.
We show the existence of a novel type of interstitial cell-telocytes (TC) in mouse trachea and lungs. We used cell cultures, vital stainings, as well as scanning electron microscopy (SEM), transmission electron microscopy (TEM) and immunohistochemistry (IHC). Phase contrast microscopy on cultured cells showed cells with unequivocally characteristic morphology of typical TC (cells with telopodes-Tp). SEM revealed typical TC with two to three Tp-very long and branched cell prolongations. Tp consist of an alternation of thin segments (podomers) and thick segments (podoms). The latter accommodate mitochondria (as shown by Janus Green and MitoTracker), rough endoplasmic reticulum and caveolae. TEM showed characteristic podomers and podoms as well as close relationships with nerve endings and blood capillaries. IHC revealed positive expression of TC for c-kit, vimentin and CD34. In conclusion, this study shows the presence in trachea and lungs of a peculiar type of cells, which fulfils the criteria for TC.
我们展示了一种新型的间充质细胞-间质细胞（TC）在小鼠气管和肺部的存在。我们使用细胞培养、活体染色以及扫描电子显微镜（SEM）、透射电子显微镜（TEM）和免疫组织化学（IHC）进行了研究。对培养细胞的相差显微镜观察显示，细胞具有典型 TC（具有 telopodes-Tp 的细胞）的明确特征形态。SEM 显示出典型的 TC，具有两到三个 Tp-非常长且分支的细胞延伸。Tp 由薄节段（podomers）和厚节段（podoms）交替组成。后者容纳线粒体（如 Janus Green 和 MitoTracker 所示）、粗面内质网和 caveolae。TEM 显示了特征性的 podomers 和 podoms 以及与神经末梢和毛细血管的密切关系。IHC 显示 TC 对 c-kit、波形蛋白和 CD34 的阳性表达。总之，这项研究表明在气管和肺部存在一种特殊类型的细胞，符合 TC 的标准。
