Osteoporosis Research Center, Creighton University, 601 N 30th St Omaha, NE 68131, USA.
Bone. 2011 Nov;49(5):955-64. doi: 10.1016/j.bone.2011.07.017. Epub 2011 Jul 23.
This review reports on proceedings of a bone histomorphometry session conducted at the Fortieth International IBMS Sun Valley Skeletal Tissue Biology Workshop held on August 1, 2010. The session was prompted by recent technical problems encountered in conducting histomorphometry on bone biopsies from humans and animals treated with anti-remodeling agents such as bisphosphonates and RANKL antibodies. These agents reduce remodeling substantially, and thus cause problems in calculating bone remodeling dynamics using in vivo fluorochrome labeling. The tissue specimens often contain few or no fluorochrome labels, and thus create statistical and other problems in analyzing variables such as mineral apposition rates, mineralizing surface and bone formation rates. The conference attendees discussed these problems and their resolutions, and the proceedings reported here summarize their discussions and recommendations.
这篇综述报告是在 2010 年 8 月 1 日举行的第四十届国际 IBMS 太阳谷骨骼组织生物学研讨会上的骨组织形态计量学会议上的报告。此次会议是由在使用抗重塑剂（如双磷酸盐和 RANKL 抗体）治疗的人类和动物的骨活检中进行骨组织形态计量学所遇到的一些最新技术问题而引发的。这些试剂会大大减少重塑，因此在用体内荧光标记物计算骨重塑动力学时会产生问题。组织标本通常很少或没有荧光标记物，因此在分析矿物质沉积率、矿化表面和骨形成率等变量时会产生统计和其他问题。会议与会者讨论了这些问题及其解决方案，这里报告的会议记录总结了他们的讨论和建议。
