Franke E D, Weinstein P P
J Parasitol. 1984 Oct;70(5):618-28.
Third-stage larvae of Dipetalonema viteae obtained from the tick vector developed to the fourth stage in several cell-free culture systems. Survival and development of larvae in a number of commercially available cell culture media, supplemented with serum and other defined and undefined components, were compared. All cultures were gassed with 5% carbon dioxide in nitrogen. Best survival, growth and development were obtained in stationary cultures containing 1:1 (v/v) mixtures of NCTC 135, either RPMI 1640 or Iscove's Modified Dulbecco's Medium, and a supplement of 20% non-heat-inactivated fetal bovine serum. The importance of the medium composition and physical environment of the culture system, for the survival, growth and development of D. viteae was demonstrated.
从蜱传播媒介获得的维氏双瓣线虫三期幼虫在几种无细胞培养系统中发育至四期。比较了幼虫在多种添加血清及其他特定和非特定成分的市售细胞培养基中的存活和发育情况。所有培养物均用含5%二氧化碳的氮气进行气体置换。在含有NCTC 135与RPMI 1640或伊斯科夫改良杜尔贝科培养基按1:1（v/v）混合，并添加20%非热灭活胎牛血清的静置培养物中，幼虫的存活、生长和发育情况最佳。这证明了培养系统的培养基组成和物理环境对维氏双瓣线虫存活、生长和发育的重要性。
