Zaraspe G, Cross J H
Southeast Asian J Trop Med Public Health. 1986 Dec;17(4):579-81.
Third-stage larvae of Wuchereria bancrofti recovered from laboratory raised Aedes togoi and Anopheles maculatus fed on a human volunteer were recovered by mass dissection methods and introduced into in vitro culture. LLC-MK2 cells were used as feeder cells, and the culture medium consisted of RPMI-1640 buffered with Hepes and sodium bicarbonate and supplemented with human AB serum. The third-stage larvae molted as early as 12 days and those surviving had all molted by 16 days. The fourth-stage parasites averaged in length from 1.4 mm to a maximum of 1.8 mm. Some larvae remained alive in culture as long as 40 days and while the worms were distorted in fixation, possible primodial cells of a spicule could be visualized in the rectal region. The cuticle also appeared to be separating in the posterior end. Although complete development was not achieved, it seems that with a continuing effort, success could be obtained using this culture system with feeder cells.
从实验室饲养的以一名人类志愿者为食的多斑按蚊和东乡伊蚊体内回收的班氏吴策线虫三期幼虫，通过大规模解剖方法回收，并引入体外培养。LLC-MK2细胞用作饲养细胞，培养基由用羟乙基哌嗪乙磺酸（Hepes）和碳酸氢钠缓冲并补充人AB血清的RPMI-1640组成。三期幼虫最早在12天蜕皮，存活的幼虫在16天时全部蜕皮。四期寄生虫平均长度为1.4毫米至最大1.8毫米。一些幼虫在培养中存活长达40天，虽然虫体在固定时变形，但在直肠区域可以看到可能的交合刺原始细胞。角质层在后端似乎也在分离。虽然没有实现完全发育，但似乎通过持续努力，使用这种带有饲养细胞的培养系统有可能获得成功。
