Liu Xiao-Fei, Zhu Jun, Ge Shao-Yang, Xia Lin-Jing, Yang Hai-Ying, Qian Yong-Tao, Ren Fa-Zheng
Key Laboratory of Functional Dairy of Chinese Ministry of Education, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
Nat Prod Commun. 2011 Jun;6(6):867-70.
The immunoactivity was evaluated of Dendrobium officinale Kimura & Migo, a Chinese herbal plant, and its crude polysaccharides. Different dosages of D. officinale and its polysaccharides were orally administered to healthy BALB/c mice. The control group was given distilled water. After 4 weeks, immune parameters, including cellular immunity (delayed-type hypersensitivity and natural killer cell activity), humoral immunity (serum hemolytic complement activity), nonspecific immunity (peritoneal macrophage phagocytosis) and interferon-gamma production by splenocytes were measured. The results showed that D. officinale and its polysaccharides can significantly enhance cellular immunity and nonspecific immunity in mice. Humoral immunity was also enhanced after oral administration of D. officinale, but the polysaccharides had no influence. Both D. officinale and its polysaccharides markedly increased IFN-gamma production by murine splenocytes. Six fractions were isolated from the polysaccharides; the molecular weight of the major fraction was 533,700 Da, and composed of mannose, glucose and rhamnose in a molar ratio of 7.3:1.3:1.0.
对中国草药铁皮石斛及其粗多糖的免疫活性进行了评估。将不同剂量的铁皮石斛及其多糖口服给予健康的BALB/c小鼠。对照组给予蒸馏水。4周后，测量免疫参数，包括细胞免疫（迟发型超敏反应和自然杀伤细胞活性）、体液免疫（血清溶血补体活性）、非特异性免疫（腹腔巨噬细胞吞噬作用）以及脾细胞产生的干扰素-γ。结果表明，铁皮石斛及其多糖可显著增强小鼠的细胞免疫和非特异性免疫。口服铁皮石斛后体液免疫也得到增强，但多糖对此无影响。铁皮石斛及其多糖均显著增加小鼠脾细胞产生的干扰素-γ。从多糖中分离出六个组分；主要组分的分子量为533,700 Da，由甘露糖、葡萄糖和鼠李糖以7.3:1.3:1.0的摩尔比组成。
