Lin Xiang, Liu Jingyi, Chung Wingyan, Sze Stephen Cho-Wing, Li Haiyan, Lao Lixing, Zhang Yanbo
School of Chinese Medicine, The University of Hong Kong, Pokfulam, Hong Kong, China.
Foshan University, Department of Pharmacy, Foshan, China.
Planta Med. 2015 Jan;81(2):130-7. doi: 10.1055/s-0034-1383411. Epub 2015 Jan 15.
Dendrobium officinale is an herbal medicine that has been clinically used to promote body fluid production. Previous works demonstrated that D. officinale polysaccharides could ameliorate symptoms of salivary secretion of patients with Sjögren's syndrome and in a respective mice model. In the present study, we investigated the underlying mechanism by which D. officinale polysaccharides activate M3 muscarinic receptors and induce extracellular calcium influx, leading to the translocation of aquaporin 5, a water channel protein, to the apical membrane of human submandibular gland epithelial cells. Enzymatic treatment of D. officinale polysaccharides suggested that they are hydrolyzed but do not permeate cell membranes. This finding supports the pharmacological activity of D. officinale polysaccharides to promote salivary secretion.
铁皮石斛是一种临床上用于促进津液生成的草药。先前的研究表明，铁皮石斛多糖可改善干燥综合征患者及相应小鼠模型的唾液分泌症状。在本研究中，我们探究了铁皮石斛多糖激活M3毒蕈碱受体并诱导细胞外钙内流，从而导致水通道蛋白5（一种水通道蛋白）转位至人下颌下腺上皮细胞顶端膜的潜在机制。对铁皮石斛多糖进行酶处理表明，它们会被水解但不会穿透细胞膜。这一发现支持了铁皮石斛多糖促进唾液分泌的药理活性。
