Li Yulong, Kang Zhihua, Zhang Xia, Sun Ping, Jiang Xiaohui, Han Zhengmin
College of Forestry, Nanjing Forestry University, Nanjing, China.
Jiangsu Academy of Agricultural Sciences, Nanjing, China.
PeerJ. 2021 Dec 6;9:e12555. doi: 10.7717/peerj.12555. eCollection 2021.
is a medicinal herbal plant with important health care value and high demand. Due to its slow growth and scarcity in nature, its yield depends on intensified cultivation while biotic and abiotic stresses were important factors that causes production loss. Orchidaceae can form association with rhizoctonias collectively, and studies have found that some orchids showed a high level of strain-species specificity to orchid mycorrhizal fungi (OMF), yet the specificity of OMF on needs to explored. In this study, the effects on of four OMF isolated from were tested. The obviously higher mass yield of the treated plants in medium and pots indicated the growth promotion effect of the fungi. Furthermore, an abiotic stress test indicated stronger drought tolerance among the treated plants. For the biotic stress test, two root rot pathogens, and , were isolated and identified from root rot of . In an inhibition test, the four OMF could resist the growth of these pathogens. studies showed that these four OMF could improve the survival rate and fresh weight and decrease the root rot rate of pathogen-inoculated seedlings. The four OMF namely; sp., sp., sp. and sp. are compatible strains for improving the growth rate of by increasing its environmental stress tolerance, providing an effective way to supply resources through artificial reproduction.
是一种具有重要保健价值且需求旺盛的药用草本植物。由于其生长缓慢且在自然界中稀缺,其产量依赖于集约化种植,而生物和非生物胁迫是导致产量损失的重要因素。兰科植物可与丝核菌共同形成共生关系,研究发现一些兰花对兰花菌根真菌(OMF)表现出高度的菌株 - 物种特异性,但OMF对[植物名称未明确]的特异性有待探索。在本研究中,测试了从[植物名称未明确]分离出的四种OMF对[植物名称未明确]的影响。在培养基和花盆中处理过的植株明显更高的生物量产量表明了真菌的促生长作用。此外,一项非生物胁迫试验表明处理过的植株具有更强的耐旱性。对于生物胁迫试验,从[植物名称未明确]的根腐病中分离并鉴定出两种根腐病原菌,[病原菌名称未明确]和[病原菌名称未明确]。在抑菌试验中,这四种OMF能够抑制这些病原菌的生长。[相关研究未明确]表明这四种OMF可以提高接种病原菌的幼苗的成活率和鲜重,并降低根腐率。这四种OMF分别为;[菌株名称未明确] sp.、[菌株名称未明确] sp.、[菌株名称未明确] sp.和[菌株名称未明确] sp.,是通过提高[植物名称未明确]对环境胁迫的耐受性来提高其生长速率的兼容菌株,为通过人工繁殖提供资源提供了有效途径。