Li Wei, Chen Chunying, Ye Chang, Wei Taotao, Zhao Yuliang, Lao Fang, Chen Zhen, Meng Huan, Gao Yuxi, Yuan Hui, Xing Genmei, Zhao Feng, Chai Zhifang, Zhang Xujia, Yang Fuyu, Han Dong, Tang Xianhua, Zhang Yingge
Laboratory for Bio-Environmental Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Nanotechnology of China & Institute of High Energy Physics, Chinese Academy of Science, Yuquan Road 19B, Beijing 100049, People's Republic of China. Graduate University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
Nanotechnology. 2008 Apr 9;19(14):145102. doi: 10.1088/0957-4484/19/14/145102. Epub 2008 Mar 4.
Manufactured fullerene nanoparticles easily enter into cells and hence have been rapidly developed for biomedical uses. However, it is generally unknown which route the nanoparticles undergo when crossing cell membranes and where they localize to the intracellular compartments. Herein we have used both microscopic imaging and biological techniques to explore the processes of C(60)(C(COOH)(2))(2) nanoparticles across cellular membranes and their intracellular translocation in 3T3 L1 and RH-35 living cells. The fullerene nanoparticles are quickly internalized by the cells and then routed to the cytoplasm with punctate localization. Upon entering the cell, they are synchronized to lysosome-like vesicles. The C(60)(C(COOH)(2))(2) nanoparticles entering cells are mainly via endocytosis with time-, temperature- and energy-dependent manners. The cellular uptake of C(60)(C(COOH)(2))(2) nanoparticles was found to be clathrin-mediated but not caveolae-mediated endocytosis. The endocytosis mechanism and the subcellular target location provide key information for the better understanding and predicting of the biomedical function of fullerene nanoparticles inside cells.
人工合成的富勒烯纳米颗粒很容易进入细胞，因此已迅速发展用于生物医学用途。然而，纳米颗粒穿过细胞膜时所经历的途径以及它们在细胞内区室中的定位通常尚不清楚。在此，我们使用显微镜成像和生物学技术来探索C(60)(C(COOH)(2))(2)纳米颗粒穿过细胞膜的过程及其在3T3 L1和RH-35活细胞中的细胞内转运。富勒烯纳米颗粒被细胞迅速内化，然后以点状定位被输送到细胞质中。进入细胞后，它们与溶酶体样囊泡同步。C(60)(C(COOH)(2))(2)纳米颗粒进入细胞主要通过内吞作用，且具有时间、温度和能量依赖性。发现C(60)(C(COOH)(2))(2)纳米颗粒的细胞摄取是网格蛋白介导的，而非小窝蛋白介导的内吞作用。内吞机制和亚细胞靶点定位为更好地理解和预测富勒烯纳米颗粒在细胞内的生物医学功能提供了关键信息。
