Świętek Małgorzata, Ma Yunn-Hwa, Wu Nian-Ping, Paruzel Aleksandra, Tokarz Waldemar, Horák Daniel
Institute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovského nám. 2, 162 06 Prague, Czech Republic.
Department of Physiology and Pharmacology, College of Medicine, Chang Gung University, Guishan, Taoyuan 33302, Taiwan.
Nanomaterials (Basel). 2022 Apr 11;12(8):1310. doi: 10.3390/nano12081310.
Coating of nanoparticles with gallates renders them antioxidant and enhances cellular internalization. In this study, (amino)silica magnetic particles modified with tannic acid (TA) and optionally with chitosan (CS) were developed, and their physicochemical properties and antioxidant activity were evaluated. The results demonstrated that the TA-modified aminosilica-coated particles, as well as the silica-coated particles with a double TA layer, exhibited high antioxidant activity, whereas the silica-coated particles with no or only a single TA layer were well-internalized by LN-229 cells. In addition, a magnet placed under the culture plates greatly increased the cellular uptake of all TA-coated magnetic nanoparticles. The coating thus had a considerable impact on nanoparticle-cell interactions and particle internalization. The TA-coated magnetic nanoparticles have great potential as intracellular carriers with preserved antioxidant activity.
用没食子酸盐包覆纳米颗粒可使其具有抗氧化性并增强细胞内化作用。在本研究中，制备了用单宁酸（TA）以及可选的壳聚糖（CS）修饰的（氨基）二氧化硅磁性颗粒，并对其理化性质和抗氧化活性进行了评估。结果表明，TA修饰的氨基二氧化硅包覆颗粒以及具有双层TA的二氧化硅包覆颗粒表现出高抗氧化活性，而没有TA层或只有单层TA的二氧化硅包覆颗粒被LN-229细胞很好地内化。此外，置于培养板下方的磁铁大大增加了所有TA包覆磁性纳米颗粒的细胞摄取。因此，包覆对纳米颗粒与细胞的相互作用以及颗粒内化有相当大的影响。TA包覆的磁性纳米颗粒作为具有保留抗氧化活性的细胞内载体具有巨大潜力。
