Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
Biochem Biophys Res Commun. 2011 Aug 19;412(1):132-5. doi: 10.1016/j.bbrc.2011.07.058. Epub 2011 Jul 27.
Circadian Clock genes are associated with the estrous cycle in female animals. Treatment with Per2 and Clock siRNAs decreased the number of granulosa cells and LHr expression in follicle-stimulating hormone FSH-treated granulosa cells. Per2 siRNA treatment did not stimulate the production of estradiol and expression of P450arom, whereas Clock siRNA treatment inhibited the production of estradiol and expression of P450arom mRNA. Per2 and Clock siRNA treatment increased and unchanged, respectively, progesterone production in FSH-treated granulosa cells. Similarly, expression of StAR mRNA was increased by Per2 siRNA and unchanged by Clock siRNA. Our data provide a new insight that Per2 and Clock have different action on ovarian granulosa cell functions.
生物钟基因与雌性动物的发情周期有关。用 Per2 和 Clock siRNA 处理会减少卵泡刺激素 FSH 处理的颗粒细胞中的颗粒细胞数量和 LHr 的表达。Per2 siRNA 处理不会刺激雌二醇的产生和 P450arom 的表达，而 Clock siRNA 处理则抑制雌二醇的产生和 P450arom mRNA 的表达。Per2 和 Clock siRNA 处理分别增加和不变 FSH 处理的颗粒细胞中的孕激素产生。同样，StAR mRNA 的表达也被 Per2 siRNA 增加，而被 Clock siRNA 不变。我们的数据提供了一个新的见解，即 Per2 和 Clock 对卵巢颗粒细胞功能有不同的作用。
