Gómez-Quispe O E, Gutiérrez-Reynoso G A, Gallegos-Cardenas A, Fumuso F G, Asparrin M, Asparrin-Del Carpio M, Jara C W, Ponce D, Miguel M, Youngs C R, Vivanco H W
Department of Animal Production, Universidad Nacional Agraria La Molina, Lima, Perú.
Faculty of Veterinary Medicine and Zootechnics, Universidad Nacional Micaela Bastidas de Apurímac, Abancay, Perú.
Iran J Vet Res. 2019 Spring;20(2):96-104.
One of the factors limiting successful processing of alpaca () semen is the viscosity of seminal plasma. The viscous nature of the collected ejaculate has hindered sperm cryopreservation as well as artificial insemination (AI) under field conditions.
The objective of this investigation was to evaluate recovery, motility, and plasma membrane integrity of alpaca spermatozoa after centrifugation in one of two different solutions at one of three different combinations of speed and time.
A total of 24 ejaculates was recovered from seven reproductively sound Huacaya males using a modified artificial vagina (AV) after training the animals for semen collection. A 2 × 3 factorial treatment arrangement was utilized for this study. Ejaculates were divided into fractions for centrifugation in one of two solutions (Tris extender or PureSperm80 density gradient solution) at one of three combinations of speed and time (492 × g for 15 min, 1968 × g for 10 min, or 4448 × g for 7 min). The experiment was replicated eight times.
Analysis revealed that centrifugation at 4448 × g for 7 min in PureSperm80 provided a high recovery rate of spermatozoa with the highest sperm motility and functional integrity of plasma membrane post-centrifugation. Results suggest that adoption of this procedure (centrifugation at 4448 × g for 7 min in PureSperm80) in the initial processing of alpaca ejaculates may enhance subsequent ability to use semen for AI and other assisted reproductive biotechnologies in this species.
限制羊驼精液成功处理的因素之一是精浆的粘度。采集的射精精液的粘性阻碍了精子冷冻保存以及野外条件下的人工授精（AI）。
本研究的目的是评估在两种不同溶液之一中，以三种不同速度和时间组合之一进行离心后，羊驼精子的回收率、活力和质膜完整性。
在训练动物进行精液采集后，使用改良的人工阴道（AV）从7只生殖功能健全的瓦卡亚雄性羊驼中总共采集了24份射精精液。本研究采用2×3析因处理设计。将射精精液分成几份分别在两种溶液（Tris稀释液或PureSperm80密度梯度溶液）之一中，以三种速度和时间组合之一（492×g离心15分钟、1968×g离心10分钟或4448×g离心7分钟）进行离心。该实验重复了8次。
分析表明，在PureSperm80溶液中以4448×g离心7分钟可使精子回收率高，且离心后精子活力最高，质膜功能完整性最佳。结果表明，在羊驼射精精液的初始处理中采用此程序（在PureSperm80溶液中以4448×g离心7分钟）可能会提高该物种后续使用精液进行人工授精和其他辅助生殖生物技术的能力。
