Bavarian Health and Food Safety Authority, Veterinärstr. 2, 85764 Oberschleißheim, Germany.
J Pharm Biomed Anal. 2011 Dec 5;56(4):705-12. doi: 10.1016/j.jpba.2011.07.012. Epub 2011 Jul 26.
A new herbal product advertised as potency pill was sent for analysis by the local authority. The product was tested for the presence of potential derivatives of PDE-5 inhibitors, such as sildenafil, vardenafil, and tadalafil. Sildenafil analogues were identified, in which the piperazine ring and the sulfonyl group were replaced by a piperazinone and a hydroxyethyl structure, respectively. The chemical structures were established by LC-MS in ESI negative mode, UV and NMR spectroscopy (including DEPT, HSQC, HMBC, H,H-COSY, H,H-TOCSY and H,H-NOESY experiments). This is the first report of piperazinonafil and isopiperazinonafil as adulterant in an herbal food supplement.
一种新的草药产品，被宣传为壮阳丸，被当地当局送去分析。该产品经过测试，发现了潜在的 PDE-5 抑制剂衍生物，如西地那非、伐地那非和他达拉非。鉴定出西地那非类似物，其中哌嗪环和磺酰基分别被哌嗪酮和羟乙基结构取代。通过 LC-MS 在 ESI 负离子模式、UV 和 NMR 光谱（包括 DEPT、HSQC、HMBC、H,H-COSY、H,H-TOCSY 和 H,H-NOESY 实验）确定了化学结构。这是哌嗪酮西地那非和异哌嗪酮西地那非作为草药食品补充剂中的掺杂物的首次报告。
